Magnetic Resonance Materials in Physics, Biology and Medicine

, Volume 26, Issue 3, pp 337–343

1H NMR analysis of choline metabolites in fine-needle-aspirate biopsies of breast cancer

Authors

  • John M. Pearce
    • Center for Imaging ResearchUniversity of Cincinnati College of Medicine
    • Department of RadiologyCincinnati Children’s Hospital Medical Center
  • Mary C. Mahoney
    • Department of RadiologyUniversity of Cincinnati College of Medicine
  • Jing-Huei Lee
    • Center for Imaging ResearchUniversity of Cincinnati College of Medicine
    • Department of Biomedical EngineeringUniversity of Cincinnati College of Medicine
    • Department of Psychiatry and Behavioral NeuroscienceUniversity of Cincinnati College of Medicine
  • Wen-Jang Chu
    • Center for Imaging ResearchUniversity of Cincinnati College of Medicine
    • Department of Psychiatry and Behavioral NeuroscienceUniversity of Cincinnati College of Medicine
  • Kim M. Cecil
    • Department of RadiologyUniversity of Cincinnati College of Medicine
    • Department of RadiologyCincinnati Children’s Hospital Medical Center
  • Stephen M. Strakowski
    • Center for Imaging ResearchUniversity of Cincinnati College of Medicine
    • Department of Psychiatry and Behavioral NeuroscienceUniversity of Cincinnati College of Medicine
    • Center for Imaging ResearchUniversity of Cincinnati College of Medicine
    • Department of Psychiatry and Behavioral NeuroscienceUniversity of Cincinnati College of Medicine
Research Article

DOI: 10.1007/s10334-012-0349-0

Cite this article as:
Pearce, J.M., Mahoney, M.C., Lee, J. et al. Magn Reson Mater Phy (2013) 26: 337. doi:10.1007/s10334-012-0349-0

Abstract

Object

The relative amounts of choline (Cho), phosphocholine (PC), and glycerophosphocholine (GPC) may be sensitive indicators of breast cancer and the degree of malignancy. Here we implement some simple modifications to a previously developed 1H NMR analysis of fine-needle-aspirate (FNA) biopsies designed to yield sufficient spectral resolution of Cho, PC, and GPC for usable relative quantitation of these metabolites.

Materials and methods

FNA biopsies of eighteen breast lesions were examined using our modified procedure for direct 1H NMR at 400 MHz. Resonances of choline metabolites and potential interferences were fit using the computer program NUTS.

Results

Quantitation of PC, GPC, and Cho relative to each other and to (phospho)creatine was obtained for eleven confirmed cases of infiltrating ductal carcinoma. Reliable results could not be obtained for the remaining cases primarily due to interference from lidocaine anesthetic.

Conclusion

Some simple modifications of a previously developed 1H NMR analysis of FNAs yielded sufficient spectral resolution of Cho, PC, and GPC to permit usable relative quantitation at 400 MHz. In 9 of the 11 quantified cases the sum of GPC and Cho exceeded 42 % of the total choline-metabolite peak area.

Keywords

PhosphocholineGlycerophosphocholineLidocaineBiopsyPhosphoethanolamine

Abbreviations

FNA

Fine needle aspirate

PBS

Phosphate buffered saline

PC

Phosphocholine

GPC

Glycerophosphocholine

Cho

Choline

PtC

Phosphatidylcholine

PL

Phospholipid

PE

Phosphoethanolamine

GPE

Glycerophosphoethanolamine

IDC

Infiltrating ductal carcinoma

ILC

Infiltrating lobular carcinoma

Copyright information

© ESMRMB 2012