The experimental research on the inhibiting impacts of RNAi on Cyclin E in breast cancer cell line
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- Li, X., Shen, N., He, W. et al. Chin. -Ger. J. Clin. Oncol. (2011) 10: 502. doi:10.1007/s10330-011-0833-4
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The aim of the study was to investigate the biological character change of breast cancer cell line by inhibiting the Cyclin E expressing level.
Human breast cancer cell line MCF-7 was transfected by Cyclin E siRNA vector pEGFP/CCNE2. SiRNA-induced silencing of Cyclin E was determined at RNA level and protein level, respectively. The proliferation of MCF-7 and its sensitivity to chemical therapy were measured by CCK-8 assay, while cell cycle was measured by flow cytometry (FCM).
The plasmid could reduce the expression of Cyclin E. The proliferation ability of MCF-7 was decreased and the sensitivity to chemical therapy was enhanced according to the inhibition of Cyclin E expression. The transfected MCF-7 was arrested at G1 phase of cell cycle.
The inhibition of Cyclin E can decrease the breast cancer cell’s growth, increase its sensation to chemical therapy and slow down its cell cycle. The Cyclin E siRNA may provide us a practical tool for further study on the gene therapy to breast cancers.