Acute myeloid leukemia cells inhibit the differentiation and maturation of dendritic cells and induce the generation of regulatory T cells
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To investigate the effects of soluble factors secreted by acute myeloid leukemia (AML) cells on the phenotypical and functional properties of DCs derived from normal mononuclear cells.
Mononuclear cells were cultured with interleukin-4 (IL-4) and granulocyte-macrophage colony-stimulating factor (GM-CSF), in the presence or absence of 24 h culture supernatants from fresh primary AML cells, to generate immature DCs. The maturation of DCs was induced by cytokines IL-1beta, IL-6, tumor necrosis factor-alpha (TNF-alpha), and prostaglandin-2 (PGE-2). The phenotypic alterations of DCs and DCs-primed CD4+ T cells were evaluated using flow cytometry. Precursor frequency (PF) was calculated to monitor the allostimulatory effects of DCs on CD4+ and CD8+ T cells.
AML cell supernatant-treated DCs showed significantly lower expression of co-stimulatory molecules CD80 and CD86, and reduced response to cytokines IL-1beta, IL-6, TNF-alpha, and PGE-2. The allostimulatory effects of AML cell supernatant-treated DCs on CD4+ and CD8+ T cells were significantly lower than those of normal mature DCs [PF: (1.8 ± 0.5)% vs. (5.2 ± 1.6)% for CD4+ T cells, (2.1 ± 0.6)% vs. (6.5 ± 2.0)% for CD8+ T cells, P < 0.01]. These AML supernatant-induced DCs could also induce allogeneic CD4+ T cells to differentiate into CD4+CD25high T cells, which had immunophenotyping characteristics of regulatory T cells, i.e. they expressed Foxp3 but not active maker CD69.
This study demonstrates that soluble factors secreted by AML cells can inhibit development and functions of DCs. In addition, AML supernatant-induced DCs can induce the generation of CD4+CD25high T cells from CD4+ T cells, which may be a mechanism of increased prevalence of CD4+CD25high regulatory T cells and immune dysfunction in AML patients.
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- Acute myeloid leukemia cells inhibit the differentiation and maturation of dendritic cells and induce the generation of regulatory T cells
The Chinese-German Journal of Clinical Oncology
Volume 7, Issue 3 , pp 164-169
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- dendritic cells
- culture supernatants
- regulatory T cells
- acute myeloid leukemia
- Author Affiliations
- 1. Department of Hematology, Anhui Provincial Hospital, Anhui Medical University, Hefei, 230001, China
- 2. Department of Hematology, Stem Cell Research and Application Center, Union Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China