Use of microsatellite marker Pi26 for rapid discrimination of five Japanese genotypes of Phytophthora infestans

Fungal Diseases

DOI: 10.1007/s10327-009-0185-x

Cite this article as:
Akino, S., Baba, Y. & Kondo, N. J Gen Plant Pathol (2009) 75: 349. doi:10.1007/s10327-009-0185-x

Abstract

Microsatellite markers were tested to rapidly discriminate five Japanese genotypes (US-1, JP-1, JP-2, JP-3, and JP-4) of Phytophthora infestans. Collected from 1958 to 2007, 111 isolates of Japanese P. infestans were examined using a fluorescent-labeled primer and capillary electrophoresis. Microsatellite marker Pi26 generated specific products for each genotype without any differences in terms of isolation area or year for a particular genotype. The Pi26 marker is a powerful tool for obtaining information on the structure of Japanese populations of P. infestans.

Keywords

Simple sequence repeatsGenotype identificationPhytophthora infestansPotato late blight

Copyright information

© The Phytopathological Society of Japan and Springer 2009

Authors and Affiliations

  1. 1.Laboratory of Plant Pathology, Graduate School of AgricultureHokkaido UniversitySapporoJapan