Journal of General Plant Pathology

, Volume 74, Issue 2, pp 171–175

Development of an assay system using thrips-mediated inoculation to evaluate resistance of Capsicum spp. to Tomato spotted wilt virus

  • Yoshikatsu Genda
  • Shinya Tsuda
  • Osamu Nunomura
  • Tadashi Ito
Viral and Viroid Diseases

DOI: 10.1007/s10327-007-0069-x

Cite this article as:
Genda, Y., Tsuda, S., Nunomura, O. et al. J Gen Plant Pathol (2008) 74: 171. doi:10.1007/s10327-007-0069-x

Abstract

Considerable losses in pepper production by Tomato spotted wilt virus (TSWV) have been reported worldwide. In breeding programs, an assay for resistance that accurately estimates field occurrence of TSWV during pepper production is critical because the virus is vector transmitted. Here, we establish an assay system of TSWV-resistant Capsicum spp. using insect-mediated inoculation within an acrylic chamber in which environmental conditions such as temperature, light intensity, and nutrient supply are controlled. This chamber enables transmission of TSWV from viruliferous plants to plants used in the resistance assay with Frankliniella occidentalis safely, quickly, and precisely.

Keywords

Frankliniella occidentalisTomato spotted wilt virusCapsicum spp.TswAcrylic chamber

Copyright information

© The Phytopathological Society of Japan and Springer 2007

Authors and Affiliations

  • Yoshikatsu Genda
    • 1
  • Shinya Tsuda
    • 2
  • Osamu Nunomura
    • 1
  • Tadashi Ito
    • 1
  1. 1.Japan Horticultural Production and Research InstituteMatsudo, ChibaJapan
  2. 2.National Agricultural Research CenterTsukuba, IbarakiJapan