Journal of General Plant Pathology

, Volume 69, Issue 4, pp 244–249

Need for flagella for complete virulence of Pseudomonas syringae pv. tabaci: genetic analysis with flagella-defective mutants ΔfliC and ΔfliD in host tobacco plants

  • Yuki Ichinose
  • Rena Shimizu
  • Yoko Ikeda
  • Fumiko Taguchi
  • Mizuri Marutani
  • Takafumi Mukaihara
  • Yoshishige Inagaki
  • Kazuhiro Toyoda
  • Tomonori Shiraishi
Bacterial and Phytoplasma Diseases

DOI: 10.1007/s10327-003-0045-z

Cite this article as:
Ichinose, Y., Shimizu, R., Ikeda, Y. et al. J Gen Plant Pathol (2003) 69: 244. doi:10.1007/s10327-003-0045-z

Abstract

The flagellins purified from Pseudomonas syringae pv. tabaci induce a hypersensitive reaction in nonhost tomato cells. To investigate the role of flagella and flagellin in the compatible interaction, we generated two types of flagella-defective mutant. The ΔfliC mutant lost the fliC gene that encodes flagellin protein, whereas the ΔfliD mutant lost the fliD gene that encodes HAP2-capping protein. The two mutants had markedly reduced ability to cause disease symptoms in tobacco leaves. Furthermore, propagation of the mutants in tobacco leaves was less than that in wild-type pv. tabaci. Compared to the inoculation with wild-type pv. tabaci, inoculation with the two mutants did not markedly induce the expression of typical defense response-related genes such as PAL and hsr203J. Complementation of each fliC and fliD gene to the corresponding deficient mutant restored motility and virulence. These results indicate that flagella of P. syringae pv. tabaci are indispensable organelles for complete virulence on host tobacco plants.

Key words

FlagellaFlagellinHypersensitive reactionPseudomonas syringaeTobacco

Copyright information

© Springer-Verlag Tokyo 2003

Authors and Affiliations

  • Yuki Ichinose
    • 1
  • Rena Shimizu
    • 1
  • Yoko Ikeda
    • 1
  • Fumiko Taguchi
    • 1
  • Mizuri Marutani
    • 1
  • Takafumi Mukaihara
    • 2
  • Yoshishige Inagaki
    • 1
  • Kazuhiro Toyoda
    • 1
  • Tomonori Shiraishi
    • 1
  1. 1.Laboratory of Plant Pathology and Genetic Engineering, Faculty of AgricultureOkayama UniversityOkayamaJapan
  2. 2.Research Institute for Biological Sciences (RIBS)OkayamaJapan