Journal of Forest Research

, Volume 10, Issue 1, pp 21-26

First online:

Growth, nitrogen fixation and mineral acquisition of Alnus sieboldiana after inoculation of Frankia together with Gigaspora margarita and Pseudomonas putida

  • Takashi YamanakaAffiliated withForestry and Forest Products Research Institute Email author 
  • , Akio AkamaAffiliated withForestry and Forest Products Research Institute
  • , Ching-Yan LiAffiliated withPNW Research Station, USDA-FS
  • , Hiroaki OkabeAffiliated withForestry and Forest Products Research Institute

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The role of tetrapartite associations among Frankia, Gigaspora margarita (an arbuscular mycorrhizal fungus), Pseudomonas putida (rhizobacterium), and Alnus sieboldiana in growth, nitrogen fixation, and mineral acquisition of A. sieboldiana was investigated. Seedlings of A. sieboldiana were inoculated with Frankia isolated from root nodules of alder, followed by inoculation of G. margarita and P. putida, and were grown for 5 months in a greenhouse. The seedlings inoculated with Frankia and G. margarita together produced the highest biomass of shoots and root nodules. Nitrogen-fixation activity, measured by acetylene reduction assay, was observed when Frankia was inoculated. The activity, on a per-nodule gram basis, decreased after G. margarita inoculation, but on a per-plant basis there was no significant difference in the activity among inoculation treatments. The mineral content in the seedlings changed after inoculation with Frankia, but not after inoculation with P. putida and/or G. margarita. The results showed a synergistic interaction among Frankia, the mycorrhizal fungus, and the rhizobacterium on the growth of A. sieboldiana.

Key words

Alnus Frankia Gigaspora Pseudomonas Tetrapartite symbiosis