Journal of Industrial Microbiology & Biotechnology

, Volume 43, Issue 11, pp 1497–1505

Biocalcification by halophilic bacteria for remediation of concrete structures in marine environment

  • Roohi Bansal
  • Navdeep Kaur Dhami
  • Abhijit Mukherjee
  • M. Sudhakara Reddy
Environmental Microbiology - Original Paper

DOI: 10.1007/s10295-016-1835-6

Cite this article as:
Bansal, R., Dhami, N.K., Mukherjee, A. et al. J Ind Microbiol Biotechnol (2016) 43: 1497. doi:10.1007/s10295-016-1835-6

Abstract

Microbial carbonate precipitation has emerged as a promising technology for remediation and restoration of concrete structures. Deterioration of reinforced concrete structures in marine environments is a major concern due to chloride-induced corrosion. In the current study, halophilic bacteria Exiguobacterium mexicanum was isolated from sea water and tested for biomineralization potential under different salt stress conditions. The growth, urease and carbonic anhydrase production significantly increased under salt stress conditions. Maximum calcium carbonate precipitation was recorded at 5 % NaCl concentration. Application of E. mexicanum on concrete specimens significantly increased the compressive strength (23.5 %) and reduced water absorption about five times under 5 % salt stress conditions compared to control specimens. SEM and XRD analysis of bacterial-treated concrete specimens confirmed the precipitation of calcite. The present study results support the potential of this technology for improving the strength and durability properties of building structures in marine environments.

Keywords

Exiguobacterium mexicanumUreaseCarbonic anhydraseCompressive strengthCalciteConcrete

Copyright information

© Society for Industrial Microbiology and Biotechnology 2016

Authors and Affiliations

  • Roohi Bansal
    • 1
  • Navdeep Kaur Dhami
    • 1
  • Abhijit Mukherjee
    • 2
  • M. Sudhakara Reddy
    • 1
  1. 1.Department of BiotechnologyThapar UniversityPatialaIndia
  2. 2.Department of Civil EngineeringCurtin UniversityBentleyAustralia