Journal of Industrial Microbiology & Biotechnology

, Volume 39, Issue 12, pp 1761–1769

Integrated bioprocess for the stereospecific production of linalool oxides from linalool with Corynespora cassiicola DSM 62475

  • Sebastian Bormann
  • Maria M. W. Etschmann
  • Marco-Antonio Mirata
  • Jens Schrader
Biocatalysis

DOI: 10.1007/s10295-012-1181-2

Cite this article as:
Bormann, S., Etschmann, M.M.W., Mirata, MA. et al. J Ind Microbiol Biotechnol (2012) 39: 1761. doi:10.1007/s10295-012-1181-2

Abstract

Linalool oxides are of interest to the flavour industry because of their lavender notes. Corynespora cassiicola DSM 62475 has been identified recently as a production organism because of high stereoselectivity and promising productivities [Mirata et al. (2008) J Agric Food Chem 56(9):3287–3296]. In this work, the stereochemistry of this biotransformation was further investigated. Predominantly (2R)-configured linalool oxide enantiomers were produced from (R)-(−)-linalool. Comparative investigations with racemic linalool suggest that predominantly (2S)-configured derivatives can be expected by using (S)-(+)-configured substrate. Substrate and product inhibited growth even at low concentrations (200 mg l−1). To avoid toxic effects and supply sufficient substrates, a substrate feeding product removal (SFPR) system based on hydrophobic adsorbers was established. Applying SFPR, productivity on the shake flask scale was increased from 80 to 490 mg l−1 day−1. Process optimisation increased productivity to 920 mg l−1 day−1 in a bioreactor with an overall product concentration of 4.600 mg l−1 linalool oxides.

Keywords

BiotransformationLinaloolLinalool oxideSubstrate feeding product removalHydrophobic adsorber

Copyright information

© Society for Industrial Microbiology and Biotechnology 2012

Authors and Affiliations

  • Sebastian Bormann
    • 1
  • Maria M. W. Etschmann
    • 1
  • Marco-Antonio Mirata
    • 1
  • Jens Schrader
    • 1
  1. 1.Biochemical EngineeringDECHEMA Research InstituteFrankfurtGermany