Journal of Industrial Microbiology & Biotechnology

, Volume 39, Issue 11, pp 1725–1729

Inhibition of acetate accumulation leads to enhanced production of (R,R)-2,3-butanediol from glycerol in Escherichia coli

  • Xiaolin Shen
  • Yuheng Lin
  • Rachit Jain
  • Qipeng Yuan
  • Yajun Yan
Short Communication

DOI: 10.1007/s10295-012-1171-4

Cite this article as:
Shen, X., Lin, Y., Jain, R. et al. J Ind Microbiol Biotechnol (2012) 39: 1725. doi:10.1007/s10295-012-1171-4

Abstract

This work describes the production of (R,R)-2,3-butanediol in Escherichia coli using glycerol by metabolic engineering approaches. The introduction of a synthetic pathway converting pyruvate to (R,R)-2,3-butanediol into wild-type E. coli strain BW25113 led to the production of (R,R)-2,3-butanediol at a titer of 3.54 g/l and a yield of 0.131 g product/g glycerol (26.7 % of theoretical maximum) with acetate (around 3.00 g/l) as the dominant by-product. We therefore evaluated the impacts of deleting the genes ackA or/and poxB that are responsible for the major by-product, acetate. This increased production of (R,R)-2,3-butanediol to 9.54 g/l with a yield of 0.333 g product/g glycerol (68.0 % of theoretical maximum) in shake flask studies. The utilization of low-priced crude glycerol to produce value-added chemicals is of great significance to the economic viability of the biodiesel industry.

Keywords

2,3-ButanediolGlycerolEscherichia coli

Copyright information

© Society for Industrial Microbiology and Biotechnology 2012

Authors and Affiliations

  • Xiaolin Shen
    • 1
  • Yuheng Lin
    • 2
  • Rachit Jain
    • 2
  • Qipeng Yuan
    • 1
  • Yajun Yan
    • 3
  1. 1.Key Laboratory of Bioprocess of BeijingBeijing University of Chemical TechnologyBeijingChina
  2. 2.Department of Biological and Agricultural EngineeringUniversity of GeorgiaAthensUSA
  3. 3.Biochemical Engineering Program, College of EngineeringUniversity of GeorgiaAthensUSA