Journal of Industrial Microbiology & Biotechnology

, Volume 38, Issue 10, pp 1657–1665

Biosynthesis of plant-specific phenylpropanoids by construction of an artificial biosynthetic pathway in Escherichia coli

  • Oksik Choi
  • Cheng-Zhu Wu
  • Sun Young Kang
  • Jong Seog Ahn
  • Tai-Boong Uhm
  • Young-Soo Hong
Original Paper

DOI: 10.1007/s10295-011-0954-3

Cite this article as:
Choi, O., Wu, CZ., Kang, S.Y. et al. J Ind Microbiol Biotechnol (2011) 38: 1657. doi:10.1007/s10295-011-0954-3

Abstract

Biological synthesis of plant secondary metabolites has attracted increasing attention due to their proven or assumed beneficial properties and health-promoting effects. Phenylpropanoids are the precursors to a range of important plant metabolites such as the secondary metabolites belonging to the flavonoid/stilbenoid class of compounds. In this study, engineered Escherichia coli containing artificial phenylpropanoid biosynthetic pathways utilizing tyrosine as the initial precursor were established for production of plant-specific metabolites such as ferulic acid, naringenin, and resveratrol. The construction of the artificial pathway utilized tyrosine ammonia lyase and 4-coumarate 3-hydroxylase from Saccharothrix espanaensis, cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor, caffeic acid O-methyltransferase and chalcone synthase from Arabidopsis thaliana, and stilbene synthase from Arachis hypogaea.

Keywords

Biosynthesis Phenylpropanoid Heterologous expression Artificial pathway 

Supplementary material

10295_2011_954_MOESM1_ESM.doc (27 kb)
Supplementary material 1 (doc 27 kb)
10295_2011_954_MOESM2_ESM.pptx (604 kb)
Supplementary material 2 (pptx 603 kb)

Copyright information

© Society for Industrial Microbiology 2011

Authors and Affiliations

  • Oksik Choi
    • 1
    • 2
  • Cheng-Zhu Wu
    • 1
  • Sun Young Kang
    • 1
  • Jong Seog Ahn
    • 1
  • Tai-Boong Uhm
    • 2
  • Young-Soo Hong
    • 1
  1. 1.Korea Research Institute of Bioscience and BiotechnologyChungbukRepublic of Korea
  2. 2.Biological SciencesChunbuk National UniversityJeollabuk-doRepublic of Korea

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