Journal of Industrial Microbiology & Biotechnology

, Volume 38, Issue 9, pp 1467–1475

A study on the pretreatment of a sugarcane bagasse sample with dilute sulfuric acid

  • Larissa Canilha
  • Victor T. O. Santos
  • George J. M. Rocha
  • João B. Almeida e Silva
  • Marco Giulietti
  • Silvio S. Silva
  • Maria G. A. Felipe
  • André Ferraz
  • Adriane M. F. Milagres
  • Walter Carvalho
Original Paper

DOI: 10.1007/s10295-010-0931-2

Cite this article as:
Canilha, L., Santos, V.T.O., Rocha, G.J.M. et al. J Ind Microbiol Biotechnol (2011) 38: 1467. doi:10.1007/s10295-010-0931-2
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Abstract

Experiments based on a 23 central composite full factorial design were carried out in 200-ml stainless-steel containers to study the pretreatment, with dilute sulfuric acid, of a sugarcane bagasse sample obtained from a local sugar–alcohol mill. The independent variables selected for study were temperature, varied from 112.5°C to 157.5°C, residence time, varied from 5.0 to 35.0 min, and sulfuric acid concentration, varied from 0.0% to 3.0% (w/v). Bagasse loading of 15% (w/w) was used in all experiments. Statistical analysis of the experimental results showed that all three independent variables significantly influenced the response variables, namely the bagasse solubilization, efficiency of xylose recovery in the hemicellulosic hydrolysate, efficiency of cellulose enzymatic saccharification, and percentages of cellulose, hemicellulose, and lignin in the pretreated solids. Temperature was the factor that influenced the response variables the most, followed by acid concentration and residence time, in that order. Although harsher pretreatment conditions promoted almost complete removal of the hemicellulosic fraction, the amount of xylose recovered in the hemicellulosic hydrolysate did not exceed 61.8% of the maximum theoretical value. Cellulose enzymatic saccharification was favored by more efficient removal of hemicellulose during the pretreatment. However, detoxification of the hemicellulosic hydrolysate was necessary for better bioconversion of the sugars to ethanol.

Keywords

LignocelluloseCompositional analysisAcid hydrolysisEnzyme hydrolysis

Copyright information

© Society for Industrial Microbiology 2011

Authors and Affiliations

  • Larissa Canilha
    • 1
  • Victor T. O. Santos
    • 1
  • George J. M. Rocha
    • 1
  • João B. Almeida e Silva
    • 1
  • Marco Giulietti
    • 2
  • Silvio S. Silva
    • 1
  • Maria G. A. Felipe
    • 1
  • André Ferraz
    • 1
  • Adriane M. F. Milagres
    • 1
  • Walter Carvalho
    • 1
  1. 1.Department of Biotechnology, Engineering College of LorenaUniversity of São PauloLorenaBrazil
  2. 2.Department of Chemical Engineering, Center of Science and TechnologyFederal University of São CarlosSão CarlosBrazil