Journal of Industrial Microbiology & Biotechnology

, Volume 38, Issue 8, pp 1001–1011

Succinic acid production from sugarcane bagasse hemicellulose hydrolysate by Actinobacillus succinogenes

Original Paper

DOI: 10.1007/s10295-010-0874-7

Cite this article as:
Borges, E.R. & Pereira, N. J Ind Microbiol Biotechnol (2011) 38: 1001. doi:10.1007/s10295-010-0874-7


Succinic acid, a four-carbon diacid, has been the focus of many research projects aimed at developing more economically viable methods of fermenting sugar-containing natural materials. Succinic acid fermentation processes also consume CO2, thereby potentially contributing to reductions in CO2 emissions. Succinic acid could also become a commodity used as an intermediate in the chemical synthesis and manufacture of synthetic resins and biodegradable polymers. Much attention has been given recently to the use of microorganisms to produce succinic acid as an alternative to chemical synthesis. We have attempted to maximize succinic acid production by Actinobacillus succinogenes using an experimental design methodology for optimizing the concentrations of the medium components. The first experiment consisted of a 24−1 fractional factorial design, and the second entailed a Central Composite Rotational Design so as to achieve optimal conditions. The optimal concentrations of nutrients predicted by the model were: NaHCO3, 10.0 g l−1; MgSO4, 3.0 g l−1; yeast extract, 2.0 g l−1; KH2PO4. 5.0 g l−1; these were experimentally validated. Under the best conversion conditions, as determined by statistical analysis, the production of succinic acid was carried out in an instrumented bioreactor using sugarcane bagasse hemicellulose hydrolysate, yielding a concentration of 22.5 g l−1.


Sugarcane bagasse Fermentation Organic acid Carbonic gas 

Supplementary material

10295_2010_874_MOESM1_ESM.xls (266 kb)
Supplementary material 1 (XLS 265 kb)
10295_2010_874_MOESM2_ESM.jpg (32 kb)
Supplementary material 2 (JPG 31.7 kb)

Copyright information

© Society for Industrial Microbiology 2010

Authors and Affiliations

  1. 1.Bioprocess Development Laboratories, School of Chemistry, Technology CenterFederal University of Rio de JaneiroRio de JaneiroBrazil

Personalised recommendations