Journal of Industrial Microbiology & Biotechnology

, Volume 37, Issue 6, pp 631–637

Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition

  • Jeong-Keun Kim
  • Mijin Kim
  • Ssang-Goo Cho
  • Myung-Kyoo Kim
  • Suhng Wook Kim
  • Young-Hee Lim
Short Communication

DOI: 10.1007/s10295-010-0722-9

Cite this article as:
Kim, J., Kim, M., Cho, S. et al. J Ind Microbiol Biotechnol (2010) 37: 631. doi:10.1007/s10295-010-0722-9

Abstract

Mulberroside A, a glycosylated stilbene, was isolated and identified from the ethanol extract of the roots of Morus alba. Oxyresveratrol, the aglycone of mulberroside A, was produced by enzymatic hydrolysis of mulberroside A using the commercial enzyme Pectinex®. Mulberroside A and oxyresveratrol showed inhibitory activity against mushroom tyrosinase with an IC50 of 53.6 and 0.49 μM, respectively. The tyrosinase inhibitory activity of oxyresveratrol was thus approximately 110-fold higher than that of mulberroside A. Inhibition kinetics showed mulberroside A to be a competitive inhibitor of mushroom tyrosinase with l-tyrosine and l-DOPA as substrate. Oxyresveratrol showed mixed inhibition and noncompetitive inhibition against l-tyrosine and l-DOPA, respectively, as substrate. The results indicate that the tyrosinase inhibitory activity of mulberroside A was greatly enhanced by the bioconversion process.

Keywords

BiotransformationMulberroside AOxyresveratrolTyrosinase inhibitor

Supplementary material

10295_2010_722_MOESM1_ESM.doc (1.3 mb)
Supplementary material 1 (DOC 1344 kb)

Copyright information

© Society for Industrial Microbiology 2010

Authors and Affiliations

  • Jeong-Keun Kim
    • 1
  • Mijin Kim
    • 1
  • Ssang-Goo Cho
    • 2
  • Myung-Kyoo Kim
    • 3
  • Suhng Wook Kim
    • 4
  • Young-Hee Lim
    • 4
  1. 1.Department of Chemical Engineering and BiotechnologyKorea Polytechnic UniversityShihung-si, Kyunggi-doSouth Korea
  2. 2.Department of Animal BiotechnologyKonkuk UniversitySeoulSouth Korea
  3. 3.Samkyung Costech Co., Ltd.Ansan City, Kyunggi-doSouth Korea
  4. 4.Department of Biomedical Science, College of Health ScienceKorea UniversitySeoulSouth Korea