Journal of Industrial Microbiology & Biotechnology

, Volume 37, Issue 3, pp 313–322

Microarray analysis of p-anisaldehyde-induced transcriptome of Saccharomyces cerevisiae

Authors

  • Lu Yu
    • Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary MedicineJilin University
  • Na Guo
    • Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary MedicineJilin University
    • Laboratory of Nutrition and Functional FoodJilin University
  • Yi Yang
    • Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary MedicineJilin University
  • Xiuping Wu
    • Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary MedicineJilin University
  • Rizeng Meng
    • Jilin Enrty-exit Inspection and Quarantine Bureau
  • Junwen Fan
    • Laboratory Animal CenterAcademy of Military Medical Sciences
  • Fa Ge
    • Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary MedicineJilin University
  • Xuelin Wang
    • Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary MedicineJilin University
    • Laboratory of Nutrition and Functional FoodJilin University
    • Key Laboratory of Zoonosis, Ministry of Education, Institute of Zoonosis, College of Animal Science and Veterinary MedicineJilin University
Original Paper

DOI: 10.1007/s10295-009-0676-y

Cite this article as:
Yu, L., Guo, N., Yang, Y. et al. J Ind Microbiol Biotechnol (2010) 37: 313. doi:10.1007/s10295-009-0676-y

Abstract

p-Anisaldehyde (4-methoxybenzaldehyde), an extract from Pimpinella anisum L. seeds, is a potential novel preservative. To reveal the possible action mechanism of p-anisaldehyde against microorganisms, yeast-based commercial oligonucleotide microarrays were used to analyze the genome-wide transcriptional changes in response to p-anisaldehyde. Quantitative real-time RT-PCR was performed for selected genes to verify the microarray results. We interpreted our microarray data with the clustering tool, T-profiler. Analysis of microarray data revealed that p-anisaldehyde induced the expression of genes related to sulphur assimilation, aromatic aldehydes metabolism, and secondary metabolism, which demonstrated that the addition of p-anisaldehyde may influence the normal metabolism of aromatic aldehydes. This genome-wide transcriptomics approach revealed first insights into the response of Saccharomyces cerevisiae (S. cerevisiae) to p-anisaldehyde challenge.

Keywords

p-AnisaldehydeAntimicrobialGene expression profileDNA microarray

Supplementary material

10295_2009_676_MOESM1_ESM.doc (1.5 mb)
(DOC 1.47 mb)

Copyright information

© Society for Industrial Microbiology 2009