Journal of Industrial Microbiology & Biotechnology

, Volume 36, Issue 10, pp 1257–1265

Exploration of geosmin synthase from Streptomyces peucetius ATCC 27952 by deletion of doxorubicin biosynthetic gene cluster

Original Paper

DOI: 10.1007/s10295-009-0605-0

Cite this article as:
Singh, B., Oh, TJ. & Sohng, J.K. J Ind Microbiol Biotechnol (2009) 36: 1257. doi:10.1007/s10295-009-0605-0

Abstract

Thorough investigation of Streptomyces peucetius ATCC 27952 genome revealed a sesquiterpene synthase, named spterp13, which encodes a putative protein of 732 amino acids with significant similarity to S. avermitilis MA-4680 (SAV2163, GeoA) and S. coelicolor A3(2) (SCO6073). The proteins encoded by SAV2163 and SCO6073 produce geosmin in the respective strains. However, the spterp13 gene seemed to be silent in S. peucetius. Deletion of the doxorubicin gene cluster from S. peucetius resulted in increased cell growth rate along with detectable production of geosmin. When we over expressed the spterp13 gene in S. peucetius DM07 under the control of an ermE* promoter, 2.4 ± 0.4-fold enhanced production of geosmin was observed.

Keywords

GeosminSesquiterpene synthaseDoxorubicinStreptomyces peucetiusOverexpression

Abbreviations

DMAPP

Dimethylallyl diphosphate

FPP

Farnesyl diphosphate

GC–MS

Gas chromatography–mass spectrometry

TLC

Thin-layer chromatography

HPLC

High-performance liquid chromatography

IPP

Isopentenyl diphosphate

LB

Luria–Bertani

ORF

Open reading frame

PCR

Polymerase chain reaction

PKS

Polyketide synthase

RT-PCR

Reverse transcription polymerase chain reaction

Copyright information

© Society for Industrial Microbiology 2009

Authors and Affiliations

  1. 1.Institute of Biomolecule Reconstruction (iBR), Department of Pharmaceutical EngineeringSunMoon UniversityAsansiRepublic of Korea