Production and partial purification of α-amylase from a novel isolate Streptomyces gulbargensis
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- Syed, D.G., Agasar, D. & Pandey, A. J Ind Microbiol Biotechnol (2009) 36: 189. doi:10.1007/s10295-008-0484-9
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Extracellular amylase production by a newly isolated alkali-thermotolerant strain Streptomyces gulbargensis DAS 131 was optimized and characterized. The highest amylase production was achieved by growing S. gulbargensis DAS 131 in media with 1% starch. Strain exhibited maximal activity at pH 9.0 and 45°C and relatively stable in alkaline conditions (pH 11). Starch and peptone were found to be the good source of carbon and nitrogen with a yield of 2,216.6 and 2,156.1 U, respectively. Maltose and maltotriose were the main end products of starch hydrolysis, indicating α-amylase activity. SDS-PAGE analysis revealed a monomeric form with a molecular weight of 55 kDa.