A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient l-lysine production

  • Masato Ikeda
  • Junko Ohnishi
  • Mikiro Hayashi
  • Satoshi Mitsuhashi
Review Paper

DOI: 10.1007/s10295-006-0104-5

Cite this article as:
Ikeda, M., Ohnishi, J., Hayashi, M. et al. J IND MICROBIOL BIOTECHNOL (2006) 33: 610. doi:10.1007/s10295-006-0104-5
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Abstract

Based on the progress in genomics, we have developed a novel approach that employs genomic information to generate an efficient amino acid producer. A comparative genomic analysis of an industrial l-lysine producer with its natural ancestor identified a variety of mutations in genes associated with l-lysine biosynthesis. Among these mutations, we identified two mutations in the relevant terminal pathways as key mutations for l-lysine production, and three mutations in central metabolism that resulted in increased titers. These five mutations when assembled in the wild-type genome led to a significant increase in both the rate of production and final l-lysine titer. Further investigations incorporated with transcriptome analysis suggested that other as yet unidentified mutations are necessary to support the l-lysine titers observed by the original production strain. Here we describe the essence of our approach for strain reconstruction, and also discuss mechanisms of l-lysine hyperproduction unraveled by combining genomics with classical strain improvement.

Keywords

Amino acid fermentation Metabolic engineering Classical mutagenesis Genomics l-lysine Corynebacterium glutamicum 

Copyright information

© Society for Industrial Microbiology 2006

Authors and Affiliations

  • Masato Ikeda
    • 1
  • Junko Ohnishi
    • 2
  • Mikiro Hayashi
    • 2
  • Satoshi Mitsuhashi
    • 2
  1. 1.Department of Bioscience and Biotechnology, Faculty of AgricultureShinshu UniversityNaganoJapan
  2. 2.Biofrontier LaboratoriesKyowa Hakko Kogyo Co., LtdTokyoJapan

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