Nitrile bioconversion by Microbacterium imperiale CBS 498-74 resting cells in batch and ultrafiltration membrane bioreactors

  • M. Cantarella
  • L. Cantarella
  • A. Gallifuoco
  • A. Spera
Special Topic: Biodeterioration

DOI: 10.1007/s10295-004-0200-3

Cite this article as:
Cantarella, M., Cantarella, L., Gallifuoco, A. et al. J IND MICROBIOL BIOTECHNOL (2006) 33: 208. doi:10.1007/s10295-004-0200-3
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Abstract

The biohydration of acrylonitrile, propionitrile and benzonitrile catalysed by the NHase activity contained in resting cells of Microbacterium imperiale CBS 498-74 was operated at 5, 10 and 20°C in laboratory-scale batch and membrane bioreactors. The bioreactions were conducted in buffered medium (50 mM Na2HPO4/NaH2PO4, pH 7.0) in the presence of distilled water or tap-water, to simulate a possible end-pipe biotreatment process. The integral bioreactor performances were studied with a cell loading (dry cell weight; DCW) varying from 0.1 mgDCW per reactor to 16 mgDCW per reactor, in order to realize near 100% bioconversion of acrylonitrile, propionitrile and benzonitrile without consistent loss of NHase activity.

Keywords

Bioconversion Nitrile hydratase Acrylonitrile Propionitrile Benzonitrile Ultrafiltration membrane bioreactors 

Copyright information

© Society for Industrial Microbiology 2005

Authors and Affiliations

  • M. Cantarella
    • 1
  • L. Cantarella
    • 2
  • A. Gallifuoco
    • 1
  • A. Spera
    • 1
  1. 1.Department of Chemistry, Chemical Engineering and MaterialsUniversity of L’AquilaL’AquilaItaly
  2. 2.Department of Industrial EngineeringUniversity of CassinoCassinoItaly

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