Detection and characterization of chitinases and other chitin-modifying enzymes

  • Michael B. Howard
  • Nathan A. Ekborg
  • Ronald M. Weiner
  • Steven W. Hutcheson
Review Paper

DOI: 10.1007/s10295-003-0096-3

Cite this article as:
Howard, M.B., Ekborg, N.A., Weiner, R.M. et al. J IND MICROBIOL BIOTECHNOL (2003) 30: 627. doi:10.1007/s10295-003-0096-3

Abstract

Multiple industrial and medical uses of chitin and its derivatives have been developed in recent years. The demand for enzymes with new or desirable properties continues to grow as additional uses of chitin, chitooligosaccharides, and chitosan become apparent. Microorganisms, the primary degraders of chitin in the environment, are a rich source of valuable chitin-modifying enzymes. This review summarizes many methods that can be used to isolate and characterize chitin-modifying enzymes including chitin depolymerases, chitodextrinases, chitin deacetylases, N-acetylglucosaminidases, chitin-binding proteins, and chitosanases. Chitin analogs, zymography, detection of reducing sugars, genomic library screening, chitooligosaccharide electrophoresis, degenerate PCR primer design, thin layer chromatography, and chitin-binding assays are discussed.

Keywords

Chitin Chitinase Chitooligosaccharide Chitosan Chitosanase 

Copyright information

© Society for Industrial Microbiology 2003

Authors and Affiliations

  • Michael B. Howard
    • 1
  • Nathan A. Ekborg
    • 1
  • Ronald M. Weiner
    • 1
    • 2
  • Steven W. Hutcheson
    • 1
  1. 1.Department of Cell Biology and Molecular GeneticsUniversity of Maryland College ParkUSA
  2. 2. Division of Molecular and Cellular BiosciencesNational Science FoundationArlingtonUSA

Personalised recommendations