Mycoscience

, Volume 53, Issue 6, pp 427–432

Survival of anaerobic fungus Caecomyces sp. in various preservation methods: a comparative study

  • Ravinder Nagpal
  • Anil Kumar Puniya
  • Jatinder Paul Sehgal
  • Kishan Singh
Full Paper

DOI: 10.1007/s10267-012-0187-y

Cite this article as:
Nagpal, R., Puniya, A.K., Sehgal, J.P. et al. Mycoscience (2012) 53: 427. doi:10.1007/s10267-012-0187-y
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Abstract

The present investigation was designed to observe the survival of the anaerobic fungus Caecomyces sp. in various routine preservation methods. Among all the treatments, cryopreservation of fungi at −70 °C with glycerol was found to be most effective for long-term maintenance (more than 90 days) of rumen fungi, followed by dimethyl sulfoxide (DMSO) and ethylene glycol (up to 60 days). In contrast, at −196 °C, DMSO showed maximum survival (more than 90 days), followed by glycerol (up to 90 days) and ethylene glycol (up to 30 days). At 39 °C, maximum survival (up to 30 days) was observed with soft agar and wheat straw; at refrigeration temperature, preservation with Orpin’s media containing straw showed maximum survival (up to 30 days).

Keywords

Anaerobic fungiCryopreservationFiber degradationMicrobial survivalRumen microflora

Copyright information

© The Mycological Society of Japan and Springer 2012

Authors and Affiliations

  • Ravinder Nagpal
    • 1
  • Anil Kumar Puniya
    • 1
  • Jatinder Paul Sehgal
    • 2
  • Kishan Singh
    • 1
  1. 1.Dairy Microbiology DivisionNational Dairy Research InstituteKarnalIndia
  2. 2.Dairy Cattle Nutrition DivisionNational Dairy Research InstituteKarnalIndia