Mycoscience

, Volume 53, Issue 6, pp 427–432

Survival of anaerobic fungus Caecomyces sp. in various preservation methods: a comparative study

Authors

    • Dairy Microbiology DivisionNational Dairy Research Institute
  • Anil Kumar Puniya
    • Dairy Microbiology DivisionNational Dairy Research Institute
  • Jatinder Paul Sehgal
    • Dairy Cattle Nutrition DivisionNational Dairy Research Institute
  • Kishan Singh
    • Dairy Microbiology DivisionNational Dairy Research Institute
Full Paper

DOI: 10.1007/s10267-012-0187-y

Cite this article as:
Nagpal, R., Puniya, A.K., Sehgal, J.P. et al. Mycoscience (2012) 53: 427. doi:10.1007/s10267-012-0187-y

Abstract

The present investigation was designed to observe the survival of the anaerobic fungus Caecomyces sp. in various routine preservation methods. Among all the treatments, cryopreservation of fungi at −70 °C with glycerol was found to be most effective for long-term maintenance (more than 90 days) of rumen fungi, followed by dimethyl sulfoxide (DMSO) and ethylene glycol (up to 60 days). In contrast, at −196 °C, DMSO showed maximum survival (more than 90 days), followed by glycerol (up to 90 days) and ethylene glycol (up to 30 days). At 39 °C, maximum survival (up to 30 days) was observed with soft agar and wheat straw; at refrigeration temperature, preservation with Orpin’s media containing straw showed maximum survival (up to 30 days).

Keywords

Anaerobic fungi Cryopreservation Fiber degradation Microbial survival Rumen microflora

Copyright information

© The Mycological Society of Japan and Springer 2012