Abstract
Plants in tropical regions experience temperature fluctuation only in non-extreme ambient temperatures. Thus, moderate changes in temperatures, which they never experience in their local environments, might be sufficient to manifest the locally hidden phenotype caused by natural mutation. To validate this hypothesis, temperature-treating experiments were performed on Capsicum accessions collected from tropical regions. Thirty-six Capsicum accessions, collected from Caribbean countries, were screened for temperature sensitivity. Similarities in their temperature sensitivities were compared with Sy-2 (C. chinense) from Seychelles, which was previously found to be a temperature-sensitive accession. Tr-13 from Trinidad & Tobago exhibited developmental abnormalities at temperatures below 24 °C. Expression of defense-related genes was induced, and salicylic acid, which is a key molecule in the plant’s defense response, accumulated in Tr-13 at temperatures below 24 °C. Tr-13 and Sy-2 appeared normal when they were grown at temperatures simulating those in Trinidad and Seychelles, respectively. Crossing Tr-13 with No. 3341 or Sy-2 revealed that the temperature-sensitive phenotype of Tr-13 was caused by a genetic mutation in the same locus as Sy-2. Plants having a temperature-sensitive phenotype that is caused by natural mutations evade artificial selection and exist as crops in specific environments, such as tropical regions.
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Acknowledgments
We thank the Caribbean Agricultural Research and Development Institute for providing the accessions from their germplasm. We also thank anonymous reviewers for their kind suggestions and comments on the revision of the manuscript. This work was supported by a Grant-in-Aid for Scientific Research [22405017] from the Japan Society for the Promotion of Science.
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Koeda, S., Hosokawa, M., Saito, H. et al. Temperature-sensitive phenotype caused by natural mutation in Capsicum latescent in two tropical regions. J Plant Res 126, 675–684 (2013). https://doi.org/10.1007/s10265-013-0564-4
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DOI: https://doi.org/10.1007/s10265-013-0564-4