Journal of Plant Research

, Volume 125, Issue 1, pp 155–164

Drought-induced proline accumulation is uninvolved with increased nitric oxide, which alleviates drought stress by decreasing transpiration in rice

Regular Paper

DOI: 10.1007/s10265-011-0417-y

Cite this article as:
Xiong, J., Zhang, L., Fu, G. et al. J Plant Res (2012) 125: 155. doi:10.1007/s10265-011-0417-y
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Abstract

Accumulation of proline is trusted to be an adaptive response of plants against drought stress, and exogenous application of nitric oxide (NO) enhances proline accumulation in Cu-treated algae. In order to investigate whether NO works as a necessary signaling molecule in drought-induced proline accumulation in rice leaves, effects of drought stress on endogenous NO content and proline accumulation were studied in rice leaves, using sodium nitroprusside (SNP, a NO donor) and 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO, a NO scavenger). The results showed that drought treatment increased both endogenous NO and proline contents in rice leaves, while foliar spray of various concentrations of SNP failed to induce proline accumulation in the leaves of well-watered rice and foliar spray of cPTIO failed to inhibit proline accumulation in the leaves of drought-stressed rice. These results indicate that increase of endogenous NO is dispensable for proline accumulation in the leaves of rice under drought stress. Further studies indicate that exogenous application of NO alleviates drought-induced water loss and ion leakage by decreasing transpiration rate of rice leaves.

Keywords

Abscisic acidOryzaSodium nitroprussideTranspiration rateWater stress

Abbreviations

ABA

Abscisic acid

CEC

Cation exchange capacity

cPTIO

2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

DAF-FM DA

4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate

DW

Dry weight

FW

Fresh weight

NO

Nitric oxide

P5CS

Δ1-Pyrroline-5-carboxylate synthetase

PPED

Photosynthetic photo flux density

SNP

Sodium nitroprusside

SW

Saturated weight

ROS

Reactive oxygen species

RWC

Relative water content

Copyright information

© The Botanical Society of Japan and Springer 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Rice BiologyChina National Rice Research InstituteHangzhouPeople’s Republic of China
  2. 2.State Key Laboratory of Plant Physiology and Biochemistry, College of Life SciencesZhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.College of Life SciencesChina Jiliang UniversityHangzhouPeople’s Republic of China