Journal of Plant Research

, Volume 119, Issue 4, pp 373–383

Gene expression profiles during heat acclimation in Arabidopsis thaliana suspension-culture cells

  • Chan Ju Lim
  • Kyung Ae Yang
  • Joon Ki Hong
  • Jin Soo Choi
  • Dea-Jin Yun
  • Jong Chan Hong
  • Woo Sik Chung
  • Sang Yeol Lee
  • Moo Je Cho
  • Chae Oh Lim
Regular Paper

DOI: 10.1007/s10265-006-0285-z

Cite this article as:
Lim, C.J., Yang, K.A., Hong, J.K. et al. J Plant Res (2006) 119: 373. doi:10.1007/s10265-006-0285-z

Abstract

Thermotolerance is induced by moderated heat acclimation. Suspension cultures of heat-acclimated Arabidopsis thaliana L. (Heynh.), ecotype Columbia, show thermotolerance against lethal heat shock (9 min, 50°C), as evidenced by a chlorophyll assay and fluorescein diacetate staining. To monitor the genome-wide transcriptome changes induced by heat acclimation at 37°C, we constructed an A. thaliana cDNA microarray containing 7,989 unique genes, and applied it to A. thaliana suspension-culture cells harvested at various times (0.5, 1, 2.5, 6, and 16 h) during heat acclimation. Data analysis revealed 165 differentially expressed genes that were grouped into ten clusters. We compared these genes with published and publicly available microarray heat-stress-related data sets in AtGenExpress. Heat-shock proteins were strongly expressed, as previously reported, and we found several of the up-regulated genes encoded detoxification and regulatory proteins. Moreover, the transcriptional induction of DREB2 (dehydration responsive element-binding factor 2) subfamily genes and COR47/rd17 under heat stress suggested cross-talk between the signaling pathways for heat and dehydration responses.

Keywords

cDNA microarray Heat acclimation A. thaliana suspension-culture cells DREB2 

Supplementary material

S1 Representative genes of Cluster 2 to 10 that are up and down regulated by heat acclimation

10265_2006_285_ESM_supp1.xls (46 kb)
(Excel 47 KB)

S2 List of genes with each motif

10265_2006_285_ESM_supp2.xls (27 kb)
(Excel 27 KB)

S3 Expression patterns of DRE/CRT motif containing genes during heat acclimation

10265_2006_285_ESM_supp3.xls (20 kb)
(Excel 21 KB)

Copyright information

© The Botanical Society of Japan and Springer-Verlag 2006

Authors and Affiliations

  • Chan Ju Lim
    • 1
  • Kyung Ae Yang
    • 1
  • Joon Ki Hong
    • 1
  • Jin Soo Choi
    • 1
  • Dea-Jin Yun
    • 1
  • Jong Chan Hong
    • 1
  • Woo Sik Chung
    • 1
  • Sang Yeol Lee
    • 1
  • Moo Je Cho
    • 1
  • Chae Oh Lim
    • 1
  1. 1.Division of Applied Life Science (BK21), Environmental Biotechnology National Core Research Center and PMBBRCGyeongsang National UniversityJinjuKorea

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