Journal of the Association for Research in Otolaryngology

, Volume 4, Issue 3, pp 339–352

Lateral Wall Histopathology and Endocochlear Potential in the Noise-Damaged Mouse Cochlea

Authors

    • Department of Otolaryngology and Communicative DisordersCleveland Clinic Foundation, Cleveland, OH 44195
    • Department of NeurosciencesCleveland Clinic Foundation, Cleveland, OH 44195
  • M. Charles Liberman
    • Eaton–Peabody LaboratoryMassachusetts Eye and Ear Infirmary, Boston, MA 02114
    • Department of Otology and LaryngologyHarvard Medical School, Boston, MA 02114
Article

DOI: 10.1007/s10162-002-3036-4

Cite this article as:
Hirose, K. & Liberman, M. JARO (2003) 4: 339. doi:10.1007/s10162-002-3036-4

Abstract

Noise exposure damages the stria and spiral ligament and may contribute to noise-induced threshold shift by altering the endocochlear potential (EP). The aim of this study was to correlate lateral wall histopathology with changes in EP and ABR thresholds. CBA/CaJ mice were exposed to octave band (8–16 kHz) noise for 2 h at intensities ranging from 94 to 116 dB SPL and evaluated 0 h to 8 weeks postexposure. EP in control mice averaged 86 and 101 mV in apical and basal turns, respectively. The 94 dB exposures caused a 40 dB temporary threshold shift (TTS), and there was with no corresponding change in EP. The 112 and 116 dB exposures caused >60 dB threshold shifts at 24 h, and EP was transiently decreased, e.g., to 21 and 27 mV in apical and basal turns after 116 dB. By 1 week postexposure, EP returned to control values in all exposure groups, although those exposed to 112 or 116 dB showed large permanent threshold shifts (PTS). Cochleas were plastic-embedded and serial-sectioned for light microscopic and ultrastructural analysis. Acute changes included degeneration of type II fibrocytes of the spiral ligament and strial edema. The strial swelling peaked at 24 h when significant EP recovery had taken place, suggesting that these changes reflect compensatory volume changes. In the chronic state, massive loss of type II fibrocytes and degeneration of strial intermediate and marginal cells was observed with drastic reduction in membrane surface area. The results suggest that EP shifts do not occur with TTS and also do not add significantly to PTS in the steady state. However, EP loss could contribute to acute threshold shifts that resolve to a PTS. EP recovery despite significant strial degeneration may be partly due to decreased transduction current caused by hair cell damage.

Keywords

acoustic trauma stria vascularis spiral ligament cochlea

INTRODUCTION

Histopathology in noise-induced hearing loss has been studied extensively in both mammalian and avian species. Although some of the earliest investigations of important structural changes in acoustic injury evaluated all structures of the inner ear, most recent attention has focused on damage to the sensory cells and neurons. It is clear that hair cell loss and stereocilia damage are fundamentally important in the etiology of chronic threshold shifts (Robertson and Johnstone 1980; Robertson 1982; Liberman and Dodds 1984; Henderson et al. 1994) and that excitotoxic damage to afferent neurons may contribute to acute threshold shifts (Robertson 1983; Puel et al. 1998). Much less is known about how damage to accessory structures, such as the stria vascularis and spiral ligament, contributes to noise-induced hearing loss in cases of either permanent threshold shift (PTS) or temporary threshold shift (TTS) caused by exposure to noise.

Although studied less extensively, structures of the lateral wall, i.e., the stria vascularis and the spiral ligament, can also be damaged by noise exposure or by aging (Liberman and Kiang 1978; Gratton and Schulte 1995; Gratton et al. 1996; Spicer et al. 1997; Ohlemiller et al. 1999; Ichimiya et al. 2000; Hequembourg and Liberman 2001). In turn, lateral wall structures, in particular, the stria vascularis, are critical in maintaining ion homeostasis of the endolymph (Offner et al. 1987; Salt et al. 1987; Wangemann 1995; Wangemann et al. 1995; Takeuchi and Ando 1998). The presence of Na/K–ATPases and Na/K/Cl cotransporters in the fibrocytes of the spiral ligament suggest that the ligament also plays a key role in the maintenance of the ionic environment and the endocochlear potential (Schulte and Adams 1989; Spicer and Schulte 1996; Crouch et al. 1997). Previous studies have shown that noise exposure can lead to reductions in the EP (Vassout 1984; Ide and Morimitsu 1990; Wang et al. 1992; Boettcher and Schmiedt 1995; Ma et al. 1995; Li et al. 1997). Given the importance of the EP to normal cochlear sensitivity (Sewell 1984), noise-induced histopathology in the lateral wall could contribute significantly to noise-induced hearing loss. If lateral wall structures do contribute to noise-induced hearing loss, then understanding the mechanisms underlying this damage could provide a novel method of intervention, independent of efforts to preserve hair cell integrity, and could potentially lead to strategies to prevent hearing loss after acoustic overstimulation.

Although previous studies have separately documented (1) noise-induced lateral wall histopathology, (2) noise-induced sensory cell damage, (3) noise-induced EP shifts, or (4) noise-induced threshold shifts, none has examined all four issues in the same ears. Similarly, no previous study has simultaneously examined all the cell types of the lateral wall which could be important to the generation and maintenance of normal EP and endolymphatic ionic composition, i.e., including both stria vascularis and spiral ligament. Thus, there are still a number of important unanswered questions, such as (1) whether EP shifts contribute to reversible threshold shifts or, alternatively, whether they appear only when the damage is severe enough to lead to a permanent loss; (2) whether noise-induced EP shifts, and the attendant threshold shifts, ever occur in ears in which the sensory cells and neurons have not been damaged; and (3) whether lateral wall degeneration correlates with loss of EP and whether EP loss is typically accompanied by visible histopathology in the lateral wall.

To address these questions, the present study assessed EP magnitude and cochlear threshold shifts, as well as light and electron microscopic histopathology of the lateral wall, in mice exposed to noise resulting in TTS in some groups and moderate to severe PTS in others. Survivals were 0 h to 8 weeks after noise exposure to provide insight into the dynamics of injury and repair.

The CBA/CaJ mouse was used as the animal model for a number of reasons. First, the genetic homogeneity of inbred strains such as CBA/CaJ is associated with less interanimal variability in the response to acoustic injury, allowing a clearer picture of injury dynamics to emerge from evaluation of groups sacrificed at a range of postexposure times (Ou et al. 2000; Yoshida et al. 2000; Wang et al. 2002). Second, the availability of interesting transgenic mice in which particular genes have been knocked out or overexpressed is a powerful tool with which to study the mechanisms of acoustic injury (Ohlemiller et al. 1999; Hakuba et al. 2000; Prosen et al. 2000). Third, our laboratory has recently completed a quantitative light microscopic evaluation of the noise-induced patterns of cellular damage and threshold shift in this mouse strain as function of postexposure survival following exposure to a wide range of sound levels (Wang et al. 2002). This background information provides a valuable overview in which to consider the fine details of morphology revealed by the type of ultrastructural analysis used in the present study.

METHODS

Experimental groups

Male CBA/CaJ mice aged 10–12 weeks were used in the present study. Animals were randomly assigned to serve as controls or as noise-exposed subjects, and two parallel groups were studied. One group of controls and noise-exposed subjects were used for electron microscopic analysis (n = 30). The second group of controls and noise-exposed animals underwent endocochlear potential measurement (n = 98). Light microscopic histological analysis was performed in selected ears of this second group to localize the insertion point of the electrodes and to determine integrity of the reticular lamina. All animal procedures were approved by the IACUC of the Massachusetts Eye and Ear Infirmary.

Noise exposure

Animals were exposed, unanesthetized, and unrestrained within cages suspended inside a reverberant box. The stimulus for noise exposure was an octave-band noise (8.0–16.0 kHz) presented at 94, 100, 106, 112, or 116 dB SPL for 2 h. The exposure stimulus was generated by a custom-made white noise source, filtered (Brickwall Filter with a 60 dB/octave slope), amplified (Crown power amplifier), and delivered (JBL compression driver) through an exponential horn fitted to a hole in the top of the sound exposure box. Sound exposure levels were measured at four positions within each cage using a 1/4 in. Bruel & Kjaer condenser microphone. Sound pressure was confirmed to vary by <0.5 dB across these measurement positions.

Anesthesia and surgical preparation

For auditory brainstem response (ABR) measurement followed by intravascular perfusion or endocochlear potential measurement, mice were anesthetized with xylazine (20 mg/kg IP) and ketamine (100 mg/kg IP).

ABR tests

ABRs were evoked with tone pips and recorded via needle electrodes inserted through the skin (vertex to ipsilateral pinna near tragus with a ground on the back near the tail). Stimuli were 5 ms pips (0.5 ms rise–fall with a cos2 onset envelope, delivered at 40/s). The response was amplified (10,000X), filtered (100 Hz – 3 kHz), and averaged with an A–D board in a LabVIEW-driven data-acquisition system. Sound level was raised in 5 dB steps from roughly 10 dB below threshold up to 80 dB SPL. At each sound level, 1024 responses were averaged (with stimulus polarity alternated). The software averager included an artifact reject feature in which response waveforms were discarded if the peak-to-peak voltage exceeded 15 µV. ABR thresholds defined as the lowest sound level at which the response peaks were clearly present were read by eye from stacked waveforms obtained at 5 dB sound pressure intervals. Thresholds typically corresponded to a level one step below that at which the peak-to-peak response amplitude began to rise.

Endocochlear potential measures

For EP recording, a tracheotomy was performed under deep anesthesia. A silver–silver chloride reference electrode was placed under the skin of the dorsum, and the animal was secured in a head holder. An incision was then made in the inferior portion of the right postauricular sulcus; the bulla was perforated allowing for exposure of the stapedial artery, basal turn, and upper turn of the cochlea. An area of bone roughly 0.5 mm × 0.5 mm was then shaved off the otic capsule with a microscalpel, which was fashioned by grinding a #6700 Beaver blade to a 0.33 mm spatulate chisel. A micropipette electrode (10–20 MNEW GREEK CHARACTER: O) filled with 150 mM KCl was advanced through the bony aperture into the spiral ligament using a motorized microdriver. The signal was amplified X10 through a Dagan cell amplifier. DC potentials were recorded via an A–D converter coupled to a desktop computer while advancing the electrode in 3 µm steps. The EP was recorded when three criteria were met:(1) a rise in potential of greater than 10 mV was observed over a distance of 6 µm or less, 2) the positive potential was maintained for 2 min at a fixed position, and (3) the potential returned to 0 when the electrode was retracted to its original position. A second recording was made in the apical turn of the same ear. The left ear of the same mouse was then tested, using the apical turn for the third recording and the basal turn for the fourth recording. The duration of the entire procedure was typically 30–40 min.

Histological preparation

Animals were endocardially perfused with 2.5% glutaraldehyde and 1.0% paraformaldehyde in a 0.065 M phosphate buffer. Both petrous temporal bones were extracted and the round and oval windows opened to allow intra labyrinthine perfusion of fixative. After overnight fixation at 4°C, cochleas were decalcified (0.1 M EDTA with 0.4% glutaraldehyde) for 3 days and then postfixed in osrnium (1% OsO4 in dH2O) for 60 min. Cochleas were dehydrated through a graded ethanol series and propylene oxide and then embedded in Araldite resin. The plastic blocks were sectioned at 40 µm in a roughly horizontal plane parallel to the spiral axis of the modiolus. Each section was mounted and coverslipped in Permount. After light microscopic analysis, selected sections were floated off slides in xylene and remounted for ultrathin sectioning. For analysis using the electron microscope, the ultrathin sections were mounted on formvar-coated slot grids, stained with lead and uranyl acetate, and examined with a Philips CM10 electron microscope.

Morphometric analysis

For each case, the cochlear spiral was reconstructed in 3D using Neurolucida® (MicroBrightField, Colchester, VT) software and tracking the heads of pillar cells as the reference point for cochlear lengths. From these 3D data, the distance from the base was computed for each section through the duct using custom software. Cochlear location was converted into frequency according to frequency-map data described by Ehret (1983) which was fit to a mathematical equation f (kHz) = 3.109 * (10(100−d)*0.0142 − 0.7719), where d = percent distance from the cochlear base.

Hair Cell Counts

A standard cytocochleogram was prepared for each ear using high-power oil-immersion objectives and Nomarski optics. In every section through the cochlear duct, the number of present and absent hair cells was assessed throughout the entire section thickness. Evaluation of both the nuclear and cuticular regions was used to make these assessments.

Stria vascularis; light microscopic analysis

The cross-sectional area of the stria vascularis was measured in four cochlear regions. In each ear, sections representing the best cross-section through each half turn of the cochlear spiral were identified from the 3D reconstructions. In each of these sections, the stria was outlined via a drawing tube with a 20X objective, and the cross-sectional area was determined by computerized planimetry.

Electron microscopic analysis

Electron micrographs of midmodiolar cross-sections through the stria, imaged at 3200×, were selected and digitized at 600 dpi. At this magnification, the entire width of the stria can be seen, although only a portion of its height (perpendicular to the basilar membrane) is visible. Using the pencil tool in Photoshop, the plasma membranes of all marginal, intermediate, and basal cells were traced within a box spanning the entire strial width and corresponding to 15 µm of strial height. The total number of pixels in each tracing was computed and divided by the number of pixels in a line 1 µm long, thus estimating the total linear distance of plasma membrane present in each micrograph.

Spiral ligament fibrocytes

An observer estimated, in every section through the cochlear duct, the percentage of remaining fibrocytes in each class (Type I, II, or IV). A quartile scale was employed (0–25%, 25–50%, 50–75%, or 75–100% of cells remaining). Estimates of cell loss were performed with a 10X or 20X objective, were based on cell nuclei, and were referred to the cell density seen in control ears. Care was taken to assess each cochlear region separately because the detailed appearance of the spiral ligament changes dramatically from base to apex in all murine strains we examined: CBA/CaJ, C57BL/6J, 129/SvEv, and FVB/N.

RESULTS

Overview of the patterns of threshold shift and damage to the organ of Corti

To put the lateral wall damage and EP shifts in proper context, it is important to summarize the pattern and degree of noise-induced threshold shifts and the damage to the organ of Corti for the noise exposures (94–116 dB) and survivals (0 h–8 weeks) used in the present study (for further details, see Wang et al. 2002). The 94 dB exposure was designed to be at the border between reversible and irreversible damage. That is, when measured at 24 h postexposure, there was a peak threshold shift of ~50 dB (data not shown) which resolved by 2 weeks postexposure to a minimal PTS of <10 dB, as illustrated in the top panel of Figure 1. All higher-level exposures produced significant to profound PTS; for example, the 112 dB exposures produced a mean peak PTS of ~60 dB for all test frequencies ≥11.3 kHz, and the 116 dB exposure group was essentially nonresponsive at all test frequencies.

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Figure 1

ABR threshold shifts and quantitative morphometry of cellular damage 2 weeks after acoustic injury. A. Mean threshold shifts (±SEM) measured by ABR after 94, 112, and 116 dB exposures re: control nonexposed littermates (n = 4 animals per group). B. Mean fractional survival (±SEM) of first row of outer hair cells for groups exposed at 112 and 116 dB (n = 4 animals per group). C. Mean estimates of cell survival in the spiral ligament and stria vascularis after 116dB exposure (n = 4 animals per group). Fibrocyte cell survival is an estimate of cells present re: control ears; strial integrity is a measure of changes in strialcross-sectional area re: control ears. Data are replotted from a previous report (Wang et al. 2002).

For exposures at SPLs ≤112 dB, there was no significant loss of inner hair cells (data not shown) and outer hair cell loss was restricted to the extreme basal turn (hook region) of the cochlea (Fig. 1B). At 116 dB, at early postexposure survivals, many ears showed a focal cochlear region in which the reticular lamina was ruptured at the junction between the outer pillar and first-row outer hair cells. As indicated in Figure 1B, this region presumably corresponds roughly to the cochlear locus tonotopically appropriate to the spectrum of the noise band. At longer survivals, this damage resolved to a similarly located focal region of inner hair cell loss (data not shown) and to virtually complete loss of all outer hair cells throughout the basal half of the cochlea (Fig. 1B).

Endocochlear potential shifts

As summarized in Figure 2, endocochlear potentials were recorded in both apical and basal turns from groups of mice exposed to either 94, 112, or 116 dB and at postexposure survival times ranging from 0 h to 8 weeks. According to 3D reconstructions of sectioned cochleas prepared after such recordings, the cochlear locations of the two penetration sites correspond to the 6 and 45 kHz regions of the cochlea. In recordings from 20 control ears, the mean EPs recorded from apical and basal loci were +86 (SEM = 2.01 mV) and +101 mV (SEM = 3.79 mV), respectively. These values are comparable to those measured in previous studies (Cable et al. 1992; Sadanaga and Morimitsu 1995; Minowa et al. 1999).

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Figure 2

Endocochlear potential (EP) shift re: control means and ABR threshold shift (TS) re: control means plotted against postexposure time for measurements at two cochlear locations. A and B. EP measurements from the second turn and first turn, respectively, each compared with ABR threshold shifts at the test frequency appropriate for that cochlear location. Animals in each group were: Control: n = 13; 116 dB/0 h: n = 8; 116 dB/24 h: n = 13; 116 dB/7 days: n = 7; 116 dB/8 weeks: n = 7; 112 dB/0 h: n = 7; 112 dB/24 h: n = 7; 94 dB/0 h: n = 6; 94 dB/24 h: n = 6.

There was no significant change in EP after 94 dB exposure, either 0 or 24 h postexposure. In Figure 2, EP values are normalized and plotted as shifts re control, to allow comparison to ABR threshold shifts measured in the same groups. Such comparison demonstrates that the TTS measured at 45 kHz at 1 day after 94 dB exposure is not a result of changes in EP.

For the 112 dB exposure, EP was reduced by 22–35 mV immediately after noise, but recovered to control values within 24 h. After 116 dB, there was a larger EP drop (65–73 mV) that recovered within ~1 week after noise. For the 112 dB exposure, the EP shift and recovery may contribute to the ABR shift and recovery occurring a few days later (Fig. 2). For the 116 dB exposure, the ABR shift exceeded the measurement capacity of the system, thus, comparison of the two recovery curves is impossible.

The magnitude of EP shift after 116 dB was strongly influenced by the disruption of the reticular lamina. As summarized above, most of the ears exposed to 116 dB showed rupture of the reticular lamina in a focal region in the middle of the cochlea (Fig. 3A); however, a minority did not. When the cochleas were separated according to this variable, there was a significant difference in magnitude and recovery time of the EP shifts. As illustrated in Figure 3B and C, mice exposed to 116 dB, that did not show reticular lamina rupture, demonstrated EP shifts similar in magnitude to those seen after 112 dB, at both 0 and 24 h after exposure.

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Figure 3

Endocochlear potential and reticular lamina integrity after exposure to 116 dB. A. Light micrograph showing the rupture of the reticular lamina (arrow) seen acutely in the tonotopic damage focus in many ears exposed at 116 dB. B and C. Mean EP (±SEM) plotted versus cochlear frequency with subjects divided into two groups: those with intact reticular lamina (RL) and those with RL rupture in the tonotopic focus. Numbers of subjects in each group were: 0 h w/RL rupture: n = 4, 0 h w/RL intact: n = 4; 24 h w/RL rupture: n = 4; 0 h w/RL intact: n = 3.

Normal morphology of lateral wall structures in the murine cochlea

The cochlear lateral wall, including spiral ligament and stria vascularis, plays an integral role in the creation of the endolymphatic potential (EP). Fibrocytes of the spiral ligament are divided into four cell types (Spicer and Schulte 1991, 1996; Slepecky 1996) based on histologic characteristics, immunostaining patterns, and general location (Fig. 4). Ultrastructural characteristics of these cells are illustrated in Figure 5. Type I fibrocytes are located adjacent to the stria vascularis and are in gap junction continuity with the basal cells of the stria vascularis (Spicer and Schulte 1996). Collagen bundles are tightly packed in this area and are associated with the type I cells. Type II fibrocytes are concentrated in the region near the spiral prominence and have a distinct ultrastructural appearance, characterized by extensive interdigitating processes that form a complex network of membrane apposition with neighboring type II cells. The type II cells are also rich in mitochondria, which set them apart from the root cells and from type I cells. The type II cells are also in gap junction continuity with type I fibrocytes and basal cells of the stria vascularis, potentially facilitating their role in potassium recycling (Spicer and Schulte 1991; Schulte and Steel 1994). The type III fibrocytes line the bony otic capsule and are oriented perpendicular to the plane of section shown in Figure 4. Type IV fibrocytes are spindle-shaped and organelle-poor and are the only fibrocyte cell type within a triangular space inferior to the basilar crest.

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Figure 4

Schematic drawing of the cochlear duct illustrates the spatial organization of the four fibrocyte classes in the spiral ligament.

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Figure 5

Ultrastructure of normal spiral ligament fibrocytes. A. Type I fibrocytes are electron-lucent, associated with collagen bundles (black arrow), and connected by gap junctions (white arrow). B. Type II fibrocytes are associated with root cells, rich in mitochondria (white arrow), and have extensive interdigitating processes (black arrow) with adjacent type II cells. C. Type III fibrocytes appear round in cross-section due to their circumferential orientation with respect to the cochlear spiral and are associated with spiraling collagen bundles (white arrow). D. Type IV fibrocytes are fusiform, contain scant granular cytoplasm, and do not have the extensive processes of the type II cells. Scale bar applies to A–D.

The stria vascularis comprises three cell types: the marginal, intermediate, and basal (Hinojosa and Rodriguez–Echandia 1966; Jahnke 1975; Anniko and Nordemar 1980; Santos–Sacchi 1982; Wangemann 1995, 1997). The ultrastructural characteristics of these three cell types are shown in Figure 6. The marginal cell comprises the epithelial lining of the endolymphatic duct and forms a tight junction barrier between the endolymph and the intrastrial compartment. The marginal cell is rich with mitochondria, contains electron dense granules, and is extensively ramified to create a large surface area. The intermediate cell is relatively electron-lucent and has fewer organelles than the marginal cell. The intermediate cells form an extensive network of processes with the marginal cells, which maximizes the surface area for ion exchange. The basal cell’s plasma membrane lines the lateral surface of the stria and forms a tight-junction barrier between the intrastrial space and spiral ligament. The basal cell cytoplasm is in gap junction continuity with type I and type II fibrocytes (Schulte and Steel 1994).

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Figure 6

Ultrastructure of the control stria vascularis. Marginal cell with its mitochondrion-rich, electron-dense cytoplasm shows extensive membrane interdigitation (e.g., white arrow) with processes of the more electron-lucent intermediate cells. Basal cells have extensive gap junctions with the underlying layer of ligament fibrocytes (black arrow).

Lateral wall histopathology: light microscopic (LM) observations

At the LM level, noise-induced changes in the lateral wall differ in acute (within 24 h postexposure) vs. chronic (2 weeks postexposure) states. Changes visible at the LM level included acute swelling and chronic shrinkage of the stria vascularis as well as acute vacuolization and chronic degeneration of the type II and type IV fibrocytes.

As shown in Figure 7, acute swelling of the stria after 116 dB exposure is remarkable, even under light microscopy. Large spaces form within the epithelium (arrow in Fig. 7C), the marginal cell nuclei are rounded and bulge into the lumen of scala media, and the vessel lumena appear dilated (compare Fig. 7A with C). LM morphometry (Wang et al. 2002) showed that acute swelling of the strial epithelium was significant for all exposure levels a ≥100 dB, i.e., for all exposures, which would ultimately result in PTS. In the chronic state, 2 weeks and more after exposure the stria vascularis is degenerated and atrophic and gaps between cells persist. LM morphometry also showed the chronic atrophy of the epithelium to be significant for all exposures ≥100 dB. The degree of strial shrinkage vs. cochlear location for the 116 dB exposures is illustrated in Figure 1C.

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Figure 7

Light microscopic changes in the lateral wall seen in the upper basal turn after exposure to 116 dB. A. Control ear. B. Eight hours after exposure, vacuoles form in the cytoplasm of type II fibrocytes (white arrow), whereas adjacent root cells appear unaffected (black arrow). C. Twenty-four hours after exposure, the stria vascularis is swollen and large intercellular spaces have formed (white arrow). D. Two weeks after overexposure, type II fibrocytes are missing in both the spiral prominence and the more inferior portion of the spiral ligament (black and white arrows), whereas type I fibrocytes appear intact. The stria is atrophic and retains excess extracellular space.

Type IV fibrocyte loss was striking under light microscopy at 1 week survival for all sound pressure levels. The loss of these cells was consistently confined to the 10–40 kHz region (Fig. 1C). At the light microscopic level, type II fibrocyte loss was detectable only in the 116 dB group and was observed in the entire the basal turn (Fig. 1C). In the acute stage, i.e., 8 h after exposure, intracytoplasmic vacuoles were widespread in type II fibrocytes (arrow in Fig. 7B). At 2 weeks postexposure, type II fibrocytes have largely disappeared, both from the spiral prominence and from the more inferior portion of the ligament (arrows in Fig. 7D).

Lateral wall histopathology: electron microscopic (EM) observations

Stria vascularis

Electron microscopy demonstrates that acute strial swelling is largely due to an increase in extracellular space between marginal and intermediate cells (Fig. 8A) and partly due to marginal cell swelling. This transient swelling of the stria is accompanied by irreversible degeneration of intermediate cells: They undergo shrinkage, darkening, and vacuolization, consistent with apoptosis (Fig. 8A). By 2 weeks postexposure (Fig. 8B), the marginal cells have lost their processes and the intermediate cells appear to be undergoing phagocytosis by cells resembling macrophages. There is evidence of dark, condensed cellular debris within the cytoplasm of larger cells with multiple lobules reminiscent of pigmented melanocytes in the control ears. There are large clefts that have formed around the small vessels, and extracellular fluid appears accumulated in the perivascular spaces and between the marginal and intermediate cell processes. The basal cells normally form a continuous layer, with tight junctions between adjacent basal cells separating the strial compartment of the lateral wall from the spiral ligament. This barrier is disrupted after acoustic trauma, and gaps are seen between basal cells in the damaged lateral wall.

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Figure 8

Ultrastructural changes in the stria vascularis after exposure to 116 dB. A. Eight hours after exposure in the upper basal turn (approximately 22 kHz region), marginal cells are swollen (black arrow), intermediate cells are shrunken and pyknotic (white arrow), and large intercellular spaces form between processes of marginal and intermediate cells. B. Two weeks after noise exposure in the lower apical turn at approximately 6 kHz, the stria is thin and atrophio, marginal cells (black arrow) lack interdigitated processes, and intermediate cells are disintegrated (white arrow). Scale bar applies to A and B.

Over the next 2–8 weeks, the stria continues to shrink and intermediate cells degenerate. There is no indication of intermediate cell recovery. Thus, the amplified membrane that normally characterizes the intermediate/marginal cell apposition is greatly reduced 2–8 weeks after noise exposure. The thinning of the stria vascular was most severe in the regions where the reticular lamina was broken and less prominent in the high-frequency region of the cochlea. The plasma membrane of the three cell types of the stria is shown in Figure 9, demonstrating the dramatic loss of cell surface area after 116 dB noise exposure compared with the control ear. In the sections traced, there is an eightfold reduction in plasma membrane elaboration in the noise-damaged ear when compared with control. Presumably, these changes in surface area led to reduced ion transport. Although this was the only region in which this type of loss was quantified, visual inspection of many micrographs suggests that such changes are typical of the lateral wall after the 116 dB exposures. The loss of membrane surface area and loss of the intermediate cells were less dramatic but still present at 112 dB and lower exposures.

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Figure 9

After 116 dB noise exposure, marginal and intermediate cells of the stria vascularis show extensive loss of membrane surface area. A and B. Tracings of all apposing membrane surfaces superimposed on electron micrographs from (A) a control stria and (B) a stria 4 weeks after 116 dB. Both micrographs are from the upper basal turn. Note that this example demonstrates a roughly 50% decrease in strial cross sectional area, whereas the mean reduction for this cochlear region was ~20% (Fig. 1C). SM = scala media; MC = marginal cell; IC = intermediate cell; BC = basal cell; nuc = nucleus; SL = spiral ligament; lumen = endothelial cell lumen. Scale bar applies to A and B.

Type II fibrocytes

From an ultrastructural perspective, type II fibrocytes showed marked histopathology immediately after exposure for all SPLs ≥100 (Fig. 10). At 0 h after 100 dB exposure (Fig. 10A), the type II cells in the spiral prominence region showed intracytoplasmic vacuolization, increased extracellular space around their processes, and breakdown of the plasma membrane. Within 24 h, there was frank rupture of the type II cell membrane, with formation of darkened fragments of cellular membrane and debris (Fig. 10B,C). The adjacent root cells remain entirely normal (Fig. 10C,D). Figure 10C demonstrates the characteristic darkening and shrinkage of type II nuclei (arrow) and intracytoplasmic vacuolization. Figure 10D is a higher-magnification view of the type II fibrocyte (arrows) flanked by root cells, highlighting the intracellular swelling that results in blebbing of the processes and disruption of the mitochondrial cristae (arrows) caused by noise exposure leaving the root cell mitochondria intact. The degeneration patterns of type II fibrocytes were suggestive of a necrotic or lytic process. The cellular debris was ultimately cleared, although phagocytic cell migration was not observed directly. Root cells appeared to sequester some cellular fragments of the type II fibrocytes without any evidence of injury to the root cells themselves.

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Figure 10

UItrastructural changes in type II fibrocytes after noise exposure. A. Zero hours after 100 dB exposure in the lower apical turn, degeneration of type II fibrocyte is seen in the spiral prominence (arrow). B. Twenty-four hours after exposure to 116 dB in the lower apical turn, cellular debris remains after lysis of type II fibrocytes (arrows). C. Eight hours after exposure to 116 dB in the upper basal turn, some type II fibrocytes are pyknotic with nuclear condensation (arrow). D. Twenty-four hours after exposure to 112 dB in the lower apical turn, mitochondrial swelling is seen within type II fibrocytes (black arrow); however, intervening root cells appear normal (white arrow). E. Two weeks after exposure to 116 dB in the lower basal turn, the spiral prominence shows loss of type II fibrocytes around the central vessel (arrow). F. Eight weeks after exposure to 116 dB in the lower apical turn, processes of type II fibrocytes are present in the spiral prominence (arrow), suggesting regeneration or repair of this cell type.

By 2 weeks postexposure, the spiral prominence is devoid of all cells except for root cell processes and vascular endothelial cells (Fig. 10E). However, 8 weeks after noise exposure, there is evidence that type II cells may have regenerated: the areas characterized by cell loss at the earlier survivals are now populated with some normal-appearing type II fibrocytes (Fig. 10F).

Type IV fibrocytes

The type IV fibrocytes also undergo degeneration, but the time course and morphology differ from that observed in the type II cells. Under light microscopy, cell loss was noted at 2 weeks postexposure. Using electron microscopy, early degenerative changes in the type IV fibrocytes were observed, but these changes were heterogeneous in nature. In some cases, type IV nuclei appeared shrunken, dark, and condensed (Fig. 11A). In other cases, the cells appeared swollen with rounded nuclei and cytoplasmic expansion and vacuolization (Fig. 11B). However, by 2 weeks postexposure, there was rampant cell loss with apoptotic bodies or cell fragments that were observed in the collagen clefts of the spiral ligament (Fig. 10C,D). These apoptotic bodies appear to be efficiently cleared during the 2–8 week period after acoustic injury. This region did not repopulate with fibrocytes during the observed period or up to 16 weeks after noise exposure.

https://static-content.springer.com/image/art%3A10.1007%2Fs10162-002-3036-4/MediaObjects/fig11.jpg
Figure 11

Ultrastructural changes in type IV fibrocytes. A. Eight hours after exposure to 116 dB in the upper basal turn, type IV fibrocytes show cytoplasmic vacuolization and cell shrinkage (arrows). B. Two weeks after exposure to 116 dB in the lower basal turn, type IV fibrocytes (e.g., at arrow) show swollen nuclei and further cytoplasmic vacuolization C and D. Eight weeks after exposure to 116 dB in the lower basal turn, some type IV fibrocytes show residual apoptotic bodies (arrows in C), shrunken remnants of cells with condensed nuclear chromatin, and open collagen clefts.

Type I and Type III fibrocytes

Neither the type I nor the type III fibrocyte was reduced in number, but the collagen bundles in close association with these cells became dispersed and appeared distended by fluid, similar to the appearance of the strial marginal and intermediate cells.

DISCUSSION

EP shifts and their contributions to temporary and permanent threshold shifts

Data from the present study are consistent with previous reports suggesting that EP shifts do not occur after noise exposures resulting in temporary threshold shifts, no matter how severe. One report (Boettcher and Schmiedt 1995) examined gerbils following repeated daily exposure to 6 h of noise at 80 dB SPL and found no significant EP changes at a postexposure time at which there was minimal TTS (~10 dB shift). Another group examined rats after a 30 min exposure to 100 dB noise and found no EP shifts at a postexposure time at which there was 20 dB of TTS (Ma et al. 1995). An early study (Benitez et al. 1972) examined a severe TTS (48 dB) measured after a 7 day exposure to an octave band of noise at 95 dB SPL and also found no EP shifts. In the present study, we examined a severe TTS (~50 dB), which is just at the border of reversibility. Thresholds measured 2 weeks postexposure to 94 dB noise (Fig. 1) show a small (but significant) permanent loss. Nevertheless, even when measured almost immediately after exposure, the EP was not significantly shifted from normal. Thus, it appears safe to conclude that the threshold elevations accompanying reversible threshold shifts, and measured in the hours and days after exposure, are not caused by any changes in EP. There may be transient changes in EP that occur during traumatic exposures; however, these changes appear to be small and to revert to baseline values within seconds or minutes after termination of the exposure (Salt and Konishi 1979; Wang et al. 1992).

There have been fewer studies of EP shifts in ears with permanent noise-induced hearing loss. One report (Ide and Morimitsu 1990), using 4 h exposures to 145 dB pure tones in guinea pig, reported a significant postexposure drop in EP, which recovered to baseline after 5 days. A study by Wang et al. (1990) demonstrated EP decrements after 20–80 min exposure to 125 dB white noise. EP recovered within 1 week postexposure, and the loss was proportional to exposure duration. However, threshold shifts were not measured in either study; thus, one can only infer, based on the sound pressure and duration of the exposures, that there was a PTS. These patterns of EP drop and recovery are similar to those in the present study (Fig. 2), showing significant EP drops following both 112 and 116 dB exposures. The initial response to 112 dB included a transient 22–35 mV drop in EP and rapid recovery during the first 24 h. Immediately after the 116 dB exposure, there was a mean EP shift of ~70 dB, which required about 7 days for full recovery. According to studies with furosemide, which decreases EP by interfering with ion transport, there is a roughly 1 dB shift in compound action potential threshold for every 1 mV decrement in EP (Sewell 1984). Thus, in cases of noise exposure that will result in PTS, any recovery of hearing thresholds within the first few days after exposure includes a significant contribution from recovery of the EP. However, it is clear that, in the steady state, permanent noise-induced hearing loss is not caused by alterations in EP, given that these EP shifts have consistently recovered to normal levels within 1 week after exposure.

Dynamics of cochlear damage and associated changes in EP after noise exposure

The lack of EP shift during a pure TTS, i.e., after the 94 dB exposure, correlated with the lack of visible morphological change in the stria or type II fibrocytes (Wang et al. 2002). On the other hand, there was massive degeneration of type IV fibrocytes throughout much of the basal turn 2 weeks following these exposures. Clearly, type IV fibrocytes are not required for normal EP generation.

For the 112 and 116 dB exposures, which caused large EP changes acutely after noise (Fig. 2), there was clearcut histopathology in many structures of the lateral wall, including strial marginal and intermediate cells as well as type II and type IV fibrocytes. The earliest changes were seen in the type II fibrocytes, which exhibited extensive cytoplasmic vacuolization immediately after these noise exposures (Fig. 10), i.e., at a time when the EP shifts were maximal. In contrast, changes in the stria vascularis were not dramatic at the earliest postexposure survivals. At these immediate postexposure times, the most important factor in determining the degree of EP shift appeared to be the integrity of the reticular lamina: In cases where the reticular lamina was breached, the EP changes were significantly larger than in those cases where it appeared intact under light microscopy (Fig. 3). It is not surprising that EP should be severely affected by large holes in the electrochemical barrier between endolymph and perilymph.

By 24 h postexposure, the EP had recovered completely after 112 dB noise. Even after the 116 dB exposure, EP had largely recovered, especially in those cases with intact reticular lamina (Figs. 2 and 3). Paradoxically, it is at this postexposure time that swelling of the stria vascularis was at its worst (Fig. 7; and Wang et al. 2002), suggesting that the swelling reflects part of the recovery process. Strial swelling likely reflects volume expansion in response to an osmotic challenge arising from increased solute load, i.e., potassium flux. This flux must be largely due to the frank ruptures of the reticular lamina (Fig. 3), but may also be due to increased flux through transduction channels during acoustic overexposure. Strial marginal cells have been noted to be primary osmoregulators and not effective volume regulators and, thus, are susceptible to large shifts in volume in response to changes in osmolarity (Wangemann et al. 2002). Previous ultrastructural investigations (Ide and Morimitsu 1990) have also noted extracellular edema and separation of marginal and intermediate cells at survival times ≤24 h.

By 2 weeks after noise exposure, there were few type II fibrocytes remaining in the spiral ligament (Fig. 7D) and the stria vascularis was atrophic throughout much the cochlea (Fig. 8B). Based on estimates made in the lower basal turn of one ear exposed to 116 dB, the membrane area of interdigitations between marginal and intermediate cells, where much of the ion pumping capacity of the stria is thought to reside, had decreased roughly an order of magnitude (Figs. 8 and 9). Despite this dramatic and widespread damage to lateral wall structures throughout the basal turn, the EP had returned to normal values, both in the apical and in the basal turns (Fig. 2).

Previous work has also suggested there is redundancy in strial pumping capacity. For example, strial atrophy in the aging gerbil ear can cause up to 60% reduction in cross-sectional area, yet be associated with <20 mV reductions in EP (Schulte and Schmiedt 1992). In the present study, a further contributing factor may be the widespread loss of hair cells and damage to stereocilia at 116 dB. The silencing of transduction associated with these structural changes eliminates a large source of transepithelial currents in the ear. This may, in turn, make it easier to maintain a normal EP with what must be dramatically reduced pumping capacity in the lateral wall.

At 8 weeks postexposure, data from the present study suggested that there might be regeneration of cells in the lateral wall, particularly among the type II fibrocytes (Fig. 10). Repopulation of cells in the stria vascularis or type IV fibrocyte region was not observed in this study, but the type II fibrocytes, which were decimated in the spiral prominence at 2 weeks, appear to be present, with extensive interdigitating processes, at 8 weeks. Evidence for proliferation of several lateral wall cell types was offered in a previous study of noise damage in the gerbil (Roberson and Rubel 1994) in which tritiated thymidine was used to assay cell division in the cochlea. There were increased numbers of labeled cells in the spiral ligament, in both the type II and the type IV fibrocyte regions, as well as in the stria vascularis. Nonetheless, the impact on recovery of hearing function would be minimal given widespread damage to sensory cells and the fact that that neither repopulation of sensory cells nor recovery of lost or fused stereocilia has been observed in this setting.

In summary, the present data suggest that lateral wall damage plays a minor role in shaping the magnitude of hearing loss in either temporary or permanent noise-induced hearing loss, primarily because damage to these structures is coupled with such severe damage to the organ of Corti that hearing is already severely compromised. On the other hand, selective damage to the lateral wall, in the absence of organ of Corti damage clearly can have important effects on hearing levels. For example, in a mouse model of DFN3, with targeted deletion of the BRN-4 gene, there is normal hair cell and spiral ganglion cell development associated with abnormalities in type I, II, and III fibrocytes (Minowa et al. 1999). In these mice, EP is decreased and ABR thresholds are elevated. Similarly, damage to lateral wall structures appears to be of greater functional significance in age-related hearing loss, at least in the gerbil; the slowly progressing loss of threshold sensitivity at high frequencies is associated with a progressive loss of EP and cellular integrity in the lateral wall which appears to precede damage to the sensory cells (Gratton et al. 1995). Both of these studies suggest that pathology in the stria vascularis and in the spiral ligament observed after acoustic trauma could have had more significant impact on hearing had there been preservation of the primary sensory apparatus.

Acknowledgements

Research supported by RO1 DC 00188 from the NIDCD and funds from the Department of Otology and Laryngology at the Massachusetts Eye and Ear Infirmary.

Copyright information

© Association for Research in Otolaryngology 2003