Invertebrate Neuroscience

, Volume 8, Issue 4, pp 167–175

Electrophysiological recording from parasitic nematode muscle

  • Alan P. Robertson
  • Sreekanth Puttachary
  • Samuel K. Buxton
  • Richard J. Martin
Original Paper

DOI: 10.1007/s10158-008-0080-8

Cite this article as:
Robertson, A.P., Puttachary, S., Buxton, S.K. et al. Invert Neurosci (2008) 8: 167. doi:10.1007/s10158-008-0080-8

Abstract

Infection of man and animals with parasitic nematodes is recognized as a significant global problem (McLeod in Int J Parasitol 25(11):1363–1367, 1994; Hotez et al. in N Engl J Med 357(10):1018–1027, 2007). At present control of these infections relies primarily on chemotherapy. There are a limited number of classes of anthelmintic compounds and the majority of these act on ion-channels of the parasite (Martin et al. in Parasitology 113:S137–S156, 1996). In this report, we describe electrophysiological recording techniques as applied to parasitic nematodes. The aim of this report is: (1) to promote the study of ion channels in nematodes to help further the understanding of antinematodal drug action; (2) to describe our recording equipment and experimental protocols; and (3) provide some examples of the information to be gleaned from this approach and how it can increase our understanding of these important pathogens.

Keywords

AnthelminticIon-channelPatch-clampCurrent-clampVoltage-clampElectrophysiology

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Alan P. Robertson
    • 1
  • Sreekanth Puttachary
    • 1
  • Samuel K. Buxton
    • 1
  • Richard J. Martin
    • 1
  1. 1.Department of Biomedical Sciences, College of Veterinary MedicineIowa State UniversityAmesUSA