Invertebrate Neuroscience

, Volume 5, Issue 3, pp 147–155

Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor-like subunit with homology to the Drosophila melanogaster Dβ1 subunit

Authors

  • A. K. Jones
    • MRC Functional Genetics Unit, Department of Human Anatomy and GeneticsUniversity of Oxford
  • J. Marshall
    • Brown University, Providence
  • A. D. Blake
    • Seton Hall University
  • S. D. Buckingham
    • MRC Functional Genetics Unit, Department of Human Anatomy and GeneticsUniversity of Oxford
  • M. G. Darlison
    • Department of Life Sciences, Neuroscience and Signal Transduction Laboratory, School of Biomedical and Natural Sciences, College of Science and TechnologyNottingham Trent University
    • MRC Functional Genetics Unit, Department of Human Anatomy and GeneticsUniversity of Oxford
Original Article

DOI: 10.1007/s10158-005-0007-6

Cite this article as:
Jones, A.K., Marshall, J., Blake, A.D. et al. Invert Neurosci (2005) 5: 147. doi:10.1007/s10158-005-0007-6

Abstract

The cloning, sequencing and functional expression of Sgβ1, a novel locust (Schistocerca gregaria) non-α nicotinic acetylcholine receptor (nAChR) subunit is described. This subunit shows 80% identity with the Drosophila melanogaster Dβ1 and 92% identity with the Locusta migratoria β1, non-α subunits but only 38% identity to Sgα1 (also referred to as αL1), a previously cloned S. gregaria nAChR α-subunit. When expressed in Xenopus laevis oocytes, Sgβ1 does not respond to nicotine. Responses to nicotine are observed, however, in oocytes co-expressing Sgα1 and Sgβ1, but the pharmacology is indistinguishable from that of currents produced by expressing Sgα1 alone. We conclude that either Sgβ1 does not co-assemble with Sgα1, or that it is unable to contribute to the functional properties of the receptor, in the Xenopus oocyte expression system.

Keywords

Nicotinic acetylcholine receptorLocust (Schistocerca gregaria)Sgβ1 non-α subunitXenopus oocyte expressionNicotinic receptor ligands

Copyright information

© Springer-Verlag 2005