Original Article

Journal of Infection and Chemotherapy

, Volume 18, Issue 5, pp 630-636

First online:

Prevalence of methicillin-resistant Staphylococcus aureus based on culture and PCR in inpatients at a tertiary care center in Tokyo, Japan

  • Hirokazu TaguchiAffiliated withDepartment of Emergency and Critical Care Medicine, Tokyo Medical University Email author 
  • , Tetsuya MatsumotoAffiliated withDepartment of Microbiology, Tokyo Medical University
  • , Hiroki IshikawaAffiliated withDepartment of Microbiology, Tokyo Medical University
  • , Shoichi OhtaAffiliated withDepartment of Emergency and Critical Care Medicine, Tokyo Medical University
  • , Tetsuo YukiokaAffiliated withDepartment of Emergency and Critical Care Medicine, Tokyo Medical University

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Abstract

We investigated active screening for colonization with methicillin-resistant Staphylococcus aureus (MRSA) on admission and weekly follow-up surveillance after admission to a tertiary care center (TCC) between June 2007 and 31 December 2007. Eleven percent (30/267) of patients were found to be positive for MRSA by polymerase chain reaction (PCR) and/or culture on admission; 5% (12/267) became positive during the TCC stay. The major primary diagnoses in MRSA-positive patients were pneumonia and cerebrovascular diseases. Twenty-two (52%) of 42 patients were found to be MRSA positive by both PCR and culture, compared with 19 (45%) of 42 who were PCR positive and culture negative. These findings suggest that active surveillance with PCR is highly sensitive and useful for the detection of MRSA colonization. To our knowledge, this is the first report of active surveillance of MRSA by PCR and bacterial culture in critically ill inpatients in Japan.

Keywords

Methicillin-resistant Staphylococcus aureus Real-time PCR Tertiary care center Active surveillance testing