Original Article

Journal of Infection and Chemotherapy

, Volume 17, Issue 1, pp 45-51

First online:

Linezolid-resistant Staphylococcus aureus isolated from 2006 through 2008 at six hospitals in Japan

  • Yurika Ikeda-DantsujiAffiliated withResearch Center for Anti-infectious Drugs, Kitasato Institute for Life Sciences, Kitasato University
  • , Hideaki HanakiAffiliated withResearch Center for Anti-infectious Drugs, Kitasato Institute for Life Sciences, Kitasato University Email author 
  • , Fuminori SakaiAffiliated withResearch Center for Anti-infectious Drugs, Kitasato Institute for Life Sciences, Kitasato UniversityGraduate School of Infection Control Sciences, Kitasato University
  • , Kazunori TomonoAffiliated withDepartment of Clinical Infectious Disease, Osaka University School of Medicine
  • , Yoshio TakesueAffiliated withDepartment of Infection Control and Prevention, Hyogo College of Medicine
  • , Junichi HondaAffiliated withDepartment of Infection Control, St. Mary’s Hospital
  • , Yuriko NonomiyaAffiliated withCentral Clinical Laboratory, Iwate Medical University Hospital
  • , Akira SuwabeAffiliated withCentral Clinical Laboratory, Iwate Medical University Hospital
  • , Osanori NaguraAffiliated withDepartment of Laboratory Medicine, Hamamatsu University School of Medicine
    • , Katsunori YanagiharaAffiliated withDepartment of Laboratory Medicine, Nagasaki University School of Medicine
    • , Hiroshige MikamoAffiliated withDepartment of Infection Control and Prevention, Aichi Medical University
    • , Kunihiko FukuchiAffiliated withDepartment of Laboratory Medicine, Showa University School of Medicine
    • , Mitsuo KakuAffiliated withDepartment of Infection Control and Laboratory Diagnostics, Internal Medicine, Tohoku University Graduate School of Medicine
    • , Shigeru KohnoAffiliated withThe Second Department of Internal Medicine, Nagasaki University School of Medicine
    • , Chie YanagisawaAffiliated withResearch Center for Anti-infectious Drugs, Kitasato Institute for Life Sciences, Kitasato University
    • , Taiji NakaeAffiliated withResearch Center for Anti-infectious Drugs, Kitasato Institute for Life Sciences, Kitasato University
    • , Koichiro YoshidaAffiliated withDepartment of Clinical Infectious Disease, Showa University School of Medicine
    • , Yoshihito NikiAffiliated withDepartment of Clinical Infectious Disease, Showa University School of Medicine

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Abstract

Limited use of linezolid for treating methicillin-resistant Staphylococcus aureus (MRSA) infection was approved in Japan in 2006. We report here the status of linezolid-resistant MRSAs in Japan. Eleven linezolid-resistant clinical isolates from 11 patients at six hospitals were collected from 2006 through 2008. The minimal inhibitory concentration (MIC) of linezolid in these strains varied from 8 to 64 μg/ml. All strains had at least one G2576T mutation in the chromosomal gene(s) encoding domain V of the 23S ribosomal RNA (rRNA). Chromosomal DNA encoding five copies of the domain V region was analyzed by polymerase chain reaction (PCR). Strains with the linezolid MICs of 64, 32, 16, and 8 μg/ml had the G2576T mutation(s) in four, three (or four), two, and one copy of the 23S rRNA genes, respectively. These results suggest that the level of linezolid resistance seems to be roughly correlated with the number of mutations in the genes encoding 23S rRNA. DNA samples from all 11 strains were subjected to pulsed-field gel electrophoresis and were classified into seven independent clones having >92% identity. Among the 11 patients, five had been treated with linezolid and the remainder, in two hospitals, had no history of prior linezolid use. The results suggested possible nosocomial infections by linezolid-resistant MRSA.

Keywords

Linezolid Resistance rRNA gene MRSA Nosocomial infection