Sources of organic matter for intertidal consumers on Ascophyllum-shores (SW Iceland): a multi-stable isotope approach
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- Sarà, G., De Pirro, M., Romano, C. et al. Helgol Mar Res (2007) 61: 297. doi:10.1007/s10152-007-0078-3
Stable isotopes were used to examine the origin of organic matter in Icelandic Ascophyllum-based habitats, the role of different organic matters in filling intertidal food webs and the food preferences of the most abundant suspension feeders, grazers and predators. We selected three intertidal sites on the SW coast of Iceland where we sampled in early September 2004, organic matter sources (POM, SOM and most abundant primary producers, A. nodosum and F. vesciculosus) and the most abundant macrofauna species (barnacles, mussels, gastropods, sponge and crabs). Even though the primary production (Ascophyllum-based) was the same at the three study sites, the isotopic composition of common-among-sites organisms varied due to local differences in the origin of available POM and SOM and in food web structures.
KeywordsBenthic organismIntertidal environmentStable isotopeIcelandNorth Atlantic
The intertidal habitats of the North Atlantic rocky seashores are among the most studied environments in the northern hemisphere (Raffaelli and Hawkins 1996; Widdows 2001). Their most characteristic macroalgae are Ascophyllum nodosum and Fucus spp. (Hansen and Ingólfsson 1993; Espinosa and Guerra-Garcia 2005). The food supply for benthic macrofauna is strongly influenced by tidal processes, which are responsible for the active transport of organic matter, mainly of macro-algal origin. Although ecological studies on single organisms from the northern intertidal are abundant (Tomanek and Helmuth 2002), little is known on organic matter sources for the key macrofauna species of these habitats. Some studies have revealed distinct differences in trophic pathways in these ecosystems, relating to biogeographical conditions (Ingólfsson 1992) or anthropogenic factors (Riera and Nieuwenhuize 2004). Thus, although some general ecological patterns can be recognised for the intertidal habitats of the northern hemisphere, special local conditions can lead to ecological scenarios, which vary from the norm (sensu Ingólfsson 1992). Examples are tidal Ascophyllum-based shores in polluted waters (Cole et al. 2004; Riera and Nieuwenhuize 2004), and shores under strong influence of inland waters (Page and Lastra 2003). To date, however, no data are available from very pristine environments such as those found on SW coasts of Iceland, where human action is rather limited and the tidal regime is very constant among sites. In the present study, we used the isotopic approach to investigate the trophic functioning of Icelandic rocky shore communities. Specifically, we selected three different sites to study: (1) the origin of organic matter in Ascophyllum-based habitats, (2) the role of different organic matters in filling intertidal food webs, and lastly (3) the food preferences of the most abundant suspension feeders, grazers and predators.
Materials and methods
Data collection and analysis
Samplings were carried out in early September 2004. At each study site, we selected different hard substrata (natural rocks in Osar and Hvassahraun and artificial substrate in Reykjavik harbour) abundantly colonised by Ascophyllum nodosum, which is the main primary producer in the area (Espinosa and Guerra-Garcia 2005). From each site, dominant macroalgae (Ascophyllum nodosum [Linnaeus] Le Jolis; Fucus vesciculosus Linnaeus) were collected by hand, and epiphytic material was removed from the thalli by gentle scraping. The first centimetre of sediment in the intertidal (in Osar and Hvassahraun only) was taken by small corers (∅ = 4 cm) to determine the origin of the sedimentary organic matter (SOM). Samples of seawater both from the Sandgerdi offshore area (as true marine referential signal) and each sampling site were collected in 4 l glass bottles; the water was filtered through pre-combusted (450°C, 4 h) Whatman GF/F filters for the isotopic analysis of the particulate organic matter (POM). All organisms abundantly found at all sites according to Espinosa and Guerra-Garcia (2005) were collected manually: the gastropods Littorina obtusata (Linnaeus, 1758), Buccinum sp., Linnaeus 1758, and Nucella lapillus (Linnaeus, 1758), the bivalve Mytilus edulis Linnaeus, 1758, the decapods Carcinus maenas (Linnaeus, 1758), the barnacle Semibalanus balanoides (Linnaeus, 1758), the sponge Halichondria panicea (Pallas, 1766) and starfish Leptasterias muelleri (M.Sars, 1846). Having been cleared of epiphytes, the animals were kept alive in filtered seawater for at least 24 h to allow for gut evacuation. After acidification (2N HCl), all samples (POM, SOM, primary organic matter sources and macrofauna) were dried at 60°C for several hours (from 24 to 72 h depending on substrate) and ground with a pestle and mortar (Abed-Navandi and Dworschak 2005). The isotopic analyses were performed using a Finnigan Delta-S isotope ratio mass spectrometer. Isotopic values were expressed in parts per thousand as deviations from standards (Peedee belemnite limestone for δ13C and nitrogen in air for δ15N): δ13C or δ15N = [(Rsample/Rstandard) −1] × 103, where R = 13C/12C or 15N/14N. The t-student was used to test for differences in isotopic values (Sokal and Rohlf 1981).
Results and discussion
In the Ascophyllum-based food webs of the Icelandic coasts, suspension feeders were mostly represented by bivalves and barnacles, the most abundant taxa in these environments (Espinosa and Guerra-Garcia 2005). At all three sites, mussels and barnacles relied directly on POM. M. edulis had a more depleted carbon composition (P > 0.05) at all sites (Osar δ13C = −20.1 ± 0.3‰; n = 30; Hvassahraun (δ13C = −17.1 ± 0.3‰; n = 30; Reykjavik harbour(δ13C = −19.6 ± 0.1‰; n = 30), suggesting a greater dependence on POM, than S. balanoides (Osar δ13C = −19.3 ± 0.4‰; n = 50; Hvassahraun (δ13C = −16.6 ± 0.7 ‰; n = 50; Reykjavik harbour(δ13C = −18.1 ± 0.2‰; n = 50), in which carbon appeared to be dependent on macroalgae-SOM detritus. The same pattern was also evident for nitrogen. Mussels in Osar and Hvassahraun were slightly more enriched in 15N than barnacles (7.3 ± 1.1‰ vs 7.1 ± 0.4‰, in Osar, and 8.4 ± 0.9‰ vs 8.1 ± 0.5‰, in Hvassahraun), while in Reykjavik harbour nitrogen values were significantly lower in mussels than in barnacles (7.4 ± 1.5‰ vs 9.8 ± 1.1‰; P < 0.05). Thus, organic matter of off- and nearshore marine origin represented the most important food source for both organisms, with SOM representing an important part of the assimilated fraction. However, as POM in these habitats showed mostly sedimentary signatures, it would seem that these intertidal Icelandic filter feeders ultimately rely on a detrital mixture originating from terrestrial, macro-algal, phytoplanktonic and likely microphytobenthic sources (i.e. mostly diatoms, which were not sampled in the present study). This result is consistent with recent findings, demonstrating that organic matter of sedimentary origin can be an important component in the diets of many marine suspensivores (Mann 1988; Langdon and Newell 1990; Sarà et al. 2002, 2003; Sarà 2006), and contradicting the view that phytoplankton is the greatest (or even only) dietary component in coastal habitats (Newell 1979; Loo and Rosenberg 1989). Only in Reykjavik harbour we found extended hard surfaces colonised by a further suspension feeder, the sponge Halichondria panicea. Its (δ13C value was slightly more negative (−20.0 ± 0.1‰) than the values of mussels and barnacles, while the sponge enriched in 15N (10.0 ± 0.2‰). The increased frequency of sponges in the harbour, compared to the “clean” sites, suggests the presence of a finer fraction of detritus strongly colonised by bacteria (Coma et al. 2001) as it is typically found in polluted sites. The different isotopic compositions of the sponge indicates that each sessile filter feeder consumes a broad spectrum of seston fractions in proportion to their availability (Coma et al. 2001).
The grazing function was mostly represented by the snail L. obtusata, the most abundant gastropod taxon. L. obtusata’s isotopic composition showed a strong dependence on A. nodosum-derived OM, above all for carbon (Osar δ13C = −15.2 ± 0.3‰; n = 20; Hvassahraun (δ13C = −15.9 ± 0.1‰; n = 20; Reykjavik harbour(δ13C = −15.5 ± 0.3‰; n = 20). With regard to nitrogen, however, isotopic values were quite and almost site-specific (Osar δ15N = 9.1 ± 0.7‰; n = 20; Hvassahraun (δ15N = 7.7 ± 0.9‰; n = 20; Reykjavik harbour δ15N = 9.4 ± 1.0‰; n = 20). The values of (δ13C (−15.0/−16.0‰ at all sites) showed that snails relied very strongly on A. nodosum carbon, while the wide variation of nitrogen values among sites suggests that the source of nitrogen varies as a function of local availability. Although our isotopic findings predict that snails mainly rely on Ascophyllum organic matter, L. obtusata in the Sandgerdi area has also been reported to feed on small propagules and spores of Fucus spp. (Espinosa and Guerra-Garcia 2005). In any case, our isotopic frame is consistent with the view that snails are usually specialist grazers on macroalgae and associated epiphytic components (Riera et al. 2000), which highlight a strong dependence of Littorina spp. on algae (Williams 1990; Albrecht and Reise 1994).
The other ecological function investigated in the present paper was that of benthic predators. At all study sites, the most abundant benthic predator was the green crab C. maenas. Other predators, such as the gastropods Buccinum sp., N. lapillus and the starfish Asterias sp., were infrequent in Osar and Hvassahraun, but abundant in Reykjavik harbour. These observations are consistent with the only published study on the intertidal benthos of Iceland (Espinosa and Guerra-Garcia 2005). However, we sampled neither intertidal fish (we sampled only on rocky) nor shorebirds such as plovers or sandpipers, although it is likely that shorebirds, in particular, are the ‘real’ top predators in these habitats, which are able to actively export secondary intertidal organic matter to adjacent ecosystems (Thompson et al. 1999). Among the active predators N. lapillus, normally believed to prey on mussels and snails (Hughes and Durwett 1985), showed in both Osar and Hvassahraun, an isotopic frame that was incompatible with the most commonly accepted view. Indeed, its mean δ13C values of −16.2 (±0.8‰) and mean (δ15N values of 6.7 (±0.3‰) were more compatible with a dependence on primary and sedimentary organic matter (mostly in Hvassahraun) than with a mussel diet. More research is needed to solve this evident discrepancy. Asterias sp., collected only from Reykjavik harbour, had δ13C values of −15.9 (±0.3‰; n = 3) and (δ15N values of 10.3 (±0.8‰; n = 3), while Buccinum sp. represented the top predator showing δ13C values of −15.5 (±0.2‰; n = 3) and δ15N values of 13.4 (±1.1‰; n = 3). Seastar isotopic composition was compatible with a diet based on mussels, and buccinids appeared to be strictly carnivorous (Hughes and Durwett 1985; Saier 2001). The diet of the North Atlantic species Buccinum undatum consists of polychaete worms, bivalves, sea urchins and the remains of bivalves killed by seastars (Ilano et al. 2005). The green crab appeared to be the general top predator in the absence of Buccinum sp. It is well known as a voracious carnivore, feeding on mussels, clams, snails, worms, barnacles, seaweed, isopods and other crustaceans (Hardwick-Witman 1985; le Roux et al. 1990; Smallegange and van de Meer 2003). Our results supported this view, as C. maenas (δ13C values at all sites, ranging from −16.0 (±1.1‰; n = 7) in Hvassahraun to −18.2 (±0.9‰; n = 8) in Reykjavik harbour (P < 0.05), demonstrated an assimilation of carbon from a mixture of bivalves, barnacles, snails and SOM. Green crab nitrogen signatures, similar at all sites (Osar δ15N = 10.1 ± 0.7‰; n = 7; Hvassahraun (δ15N = 10.1 ± 1.0‰; n = 7; Reykjavik harbour δ15N = 10.6 ± 1.4‰; n = 8), were also compatible both with this view and with the crab apical role in the Icelandic intertidal food webs.
The Ascophyllum-based intertidal communities of SW Iceland appear to be highly variable in trophic structure. Although the investigated sites are exposed to the same physical factors (i.e. tidal ranges, salinity and temperature) and have the same primary producers at their base, the isotopic composition of common-among-sites organisms varied as a function of local conditions. Particularly in Reykjavik harbour this composition was likely constrained by local pollution, although detailed information on the type and amount of pollution for this site is missing. However, the results are in line with recent literature data showing that organisms in coastal food webs have different isotopic signatures as a function of locality (Deegan and Garritt 1997; Riera and Nieuwenhuize 2004), and that at each site the isotopic frames can also vary on small to medium spatial scales (Jennings et al. 1997). The isotopic compositions of Icelandic organisms largely corresponded to those from other Atlantic areas, with the exception of N. lapillus (Hughes and Durwett 1985). The structure of intertidal Ascophyllum-based food webs on hard substrate in Iceland seems to be very simple, being based on a few functions, among which suspension feeding and grazing are the most frequent and best represented. Top predators are represented by a few taxa, and particularly C. maenas. At all sites, organic matter from A. nodosum, although directly exploited only by some grazing specialists, together with organic matter from F. vesciculosus, can flow along the food web, reaching the top predators via predation or scavenging. This findings support the idea that macroalgal organic matter can determine isotopic signatures of coastal organisms (Mann 1988; Page 1997), and that SOM plays a central role in these highly dynamic habitats (Pusceddu et al. 2003; Sarà 2006). Indeed, SOM represents the true collector of all types of locally produced organic matter, that due to hydrodynamics (e.g. tides, lateral drifting, water currents) can be re-distributed to the overlying biota (Pusceddu et al. 1999; Sarà 2006).
We are very grateful to Dr. Gudmundur Vidir Helgason for arrangements, help and hospitality during our stay at the Sandgerdi Marine Centre (SMC), Iceland, and for making available the invertebrate collections from the international BIOICE project (Benthic Invertebrates of Icelandic waters). Thanks are also due to our friend and colleague Dr. Haldor Palmar Haldorsson and all the staff of the SMC for their kindness and help. The stay at the SMC was supported by the programme ‘‘Improving Human Potential: Access to Research Infrastructure’’ (IHP/ARI) provided by the European Community.