Functional & Integrative Genomics

, Volume 13, Issue 2, pp 253–260

Esophageal cancer alters the expression of nuclear pore complex binding protein Hsc70 and eIF5A-1

  • Mehdi Moghanibashi
  • Ferdous Rastgar Jazii
  • Zahra-Soheila Soheili
  • Maryam Zare
  • Aliasghar Karkhane
  • Kazem Parivar
  • Parisa Mohamadynejad
Original Paper

DOI: 10.1007/s10142-013-0320-9

Cite this article as:
Moghanibashi, M., Rastgar Jazii, F., Soheili, ZS. et al. Funct Integr Genomics (2013) 13: 253. doi:10.1007/s10142-013-0320-9

Abstract

Nuclear pore complex (NPC) is the only corridor for macromolecules exchange between nucleus and cytoplasm. NPC and its components, nucleoporins, play important role in the diverse physiological processes including macromolecule exchange, chromosome segregation, apoptosis and gene expression. Recent reports also suggest involvement of nucleoporins in carcinogenesis. Applying proteomics, we analyzed expression pattern of the NPC components in a newly established esophageal cancer cell line from Persia (Iran), the high-risk region for esophageal cancer. Our results indicate overexpression of Hsc70 and downregulation of subunit alpha type-3 of proteasome, calpain small subunit 1, and eIF5A-1. Among these proteins, Hsc70 and eIF5A-1 are in direct interaction with NPC and involved in the nucleocytoplasmic exchange. Hsc70 plays a critical role as a chaperone in the formation of a cargo–receptor complex in nucleocytoplasmic transport. On the other hand, it is an NPC-associated protein that binds to nucleoporins and contributes in recycling of the nucleocytoplasmic transport receptors in mammals and affects transport of proteins between nucleus and cytoplasm. The other nuclear pore interacting protein: eIF5A-1 binds to the several nucleoporins and participates in nucleocytoplasmic transport. Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis.

Keywords

Nuclear pore complex (NPC) Proteomics Esophageal squamous cell carcinoma (ESCC) Esophageal cancer Hsc70 eIF5A-1 

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Mehdi Moghanibashi
    • 1
  • Ferdous Rastgar Jazii
    • 2
    • 3
  • Zahra-Soheila Soheili
    • 2
  • Maryam Zare
    • 4
  • Aliasghar Karkhane
    • 2
  • Kazem Parivar
    • 1
  • Parisa Mohamadynejad
    • 5
  1. 1.Department of Biology, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of BiochemistryNational Institute of Genetic Engineering and Biotechnology (NIGEB)TehranIran
  3. 3.Department of Pharmacology, College of MedicineUniversity of SaskatchewanSaskatoonCanada
  4. 4.Department of BiologyPayam-Noor UniversityTehranIran
  5. 5.Biotechnology Research Center, Shahrekord BranchIslamic Azad UniversityShahrekordIran

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