Functional & Integrative Genomics

, Volume 12, Issue 3, pp 515–531

Auxin and ABA act as central regulators of developmental networks associated with paradormancy in Canada thistle (Cirsium arvense)

  • James V. Anderson
  • Münevver Doğramacı
  • David P. Horvath
  • Michael E. Foley
  • Wun S. Chao
  • Jeffrey C. Suttle
  • Jyothi Thimmapuram
  • Alvaro G. Hernandez
  • Shahjahan Ali
  • Mark A. Mikel
Original Paper

DOI: 10.1007/s10142-012-0280-5

Cite this article as:
Anderson, J.V., Doğramacı, M., Horvath, D.P. et al. Funct Integr Genomics (2012) 12: 515. doi:10.1007/s10142-012-0280-5

Abstract

Dormancy in underground vegetative buds of Canada thistle, an herbaceous perennial weed, allows escape from current control methods and contributes to its invasive nature. In this study, ∼65 % of root sections obtained from greenhouse propagated Canada thistle produced new vegetative shoots by 14 days post-sectioning. RNA samples obtained from sectioned roots incubated 0, 24, 48, and 72 h at 25°C under 16:8 h light–dark conditions were used to construct four MID-tagged cDNA libraries. Analysis of in silico data obtained using Roche 454 GS-FLX pyrosequencing technologies identified molecular networks associated with paradormancy release in underground vegetative buds of Canada thistle. Sequencing of two replicate plates produced ∼2.5 million ESTs with an average read length of 362 bases. These ESTs assembled into 67358 unique sequences (21777 contigs and 45581 singlets) and annotation against the Arabidopsis database identified 15232 unigenes. Among the 15232 unigenes, we identified processes enriched with transcripts involved in plant hormone signaling networks. To follow-up on these results, we examined hormone profiles in roots, which identified changes in abscisic acid (ABA) and ABA metabolites, auxins, and cytokinins post-sectioning. Transcriptome and hormone profiling data suggest that interaction between auxin- and ABA-signaling regulate paradormancy maintenance and release in underground adventitious buds of Canada thistle. Our proposed model shows that sectioning-induced changes in polar auxin transport alters ABA metabolism and signaling, which further impacts gibberellic acid signaling involving interactions between ABA and FUSCA3. Here we report that reduced auxin and ABA-signaling, in conjunction with increased cytokinin biosynthesis post-sectioning supports a model where interactions among hormones drives molecular networks leading to cell division, differentiation, and vegetative outgrowth.

Keywords

Canada thistleDormancyESTsHormone profiling

Supplementary material

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Copyright information

© Springer-Verlag (outside the USA) 2012

Authors and Affiliations

  • James V. Anderson
    • 1
  • Münevver Doğramacı
    • 1
  • David P. Horvath
    • 1
  • Michael E. Foley
    • 1
  • Wun S. Chao
    • 1
  • Jeffrey C. Suttle
    • 2
  • Jyothi Thimmapuram
    • 3
  • Alvaro G. Hernandez
    • 3
  • Shahjahan Ali
    • 4
  • Mark A. Mikel
    • 5
  1. 1.USDA-Agricultural Research ServiceBiosciences Research LaboratoryFargoUSA
  2. 2.Northern Crop Science LaboratoryUSDA-ARSFargoUSA
  3. 3.W.M. Keck Center for Comparative and Functional GenomicsUniversity of IllinoisUrbanaUSA
  4. 4.Biosciences Core Laboratory-GenomicsUniversity of Science and Technology (KAUST)ThuwalKingdom of Saudi Arabia
  5. 5.Department of Crop Sciences and Roy J. Carver Biotechnology CenterUniversity of IllinoisUrbanaUSA