Functional & Integrative Genomics

, Volume 12, Issue 2, pp 327–339

Identification of novel and conserved Populus tomentosa microRNA as components of a response to water stress

  • Yuanyuan Ren
  • Lei Chen
  • Yiyun Zhang
  • Xiangyang Kang
  • Zhiyi Zhang
  • Yanwei Wang
Original Paper

DOI: 10.1007/s10142-012-0271-6

Cite this article as:
Ren, Y., Chen, L., Zhang, Y. et al. Funct Integr Genomics (2012) 12: 327. doi:10.1007/s10142-012-0271-6

Abstract

MicroRNAs (miRNAs) are a class of small, non-coding RNAs that play important downregulation roles in plants growth, development, and stress responses. To better identify Populus tomentosa miRNAs and understand the functions of miRNAs in response to water stress (drought and flooding), 152 conserved miRNAs belonging to 36 miRNA families, 8 known but non-conserved miRNAs and 64 candidate novel miRNAs belonging to 54 miRNA families were identified and analyzed from three small RNA (sRNA) libraries (drought treatment, flooding treatment, and control) by high-throughput sequencing combined with qRT-PCR. Significant changes in the expression of 17 conserved miRNA families and nine novel miRNAs were observed in response to drought stress, and in seven conserved miRNA families and five novel miRNAs in response to flooding stress. Both miRNA and miRNA*s were involved in the regulation of plant stress responses. The annotation of the potential targets of miRNAs with differential expression indicate that many types of genes encoding transcription factors, enzymes, and signal transduction components are implicated in the abiotic stress response..

Keywords

MicroRNA Populus tomentosa Drought Flooding High-throughput sequencing 

Supplementary material

10142_2012_271_MOESM1_ESM.pdf (2.2 mb)
ESM 1(PDF 2.20 mb)
10142_2012_271_MOESM2_ESM.doc (34 kb)
ESM 2(DOC 34 kb)
10142_2012_271_MOESM3_ESM.xls (58 kb)
ESM 3(XLS 58.0 kb)
10142_2012_271_MOESM4_ESM.xls (25 kb)
ESM 4(XLS 25 kb)
10142_2012_271_MOESM5_ESM.xls (38 kb)
ESM 5(XLS 38 kb)
10142_2012_271_MOESM6_ESM.xls (34 kb)
ESM 6(XLS 33 kb)

Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • Yuanyuan Ren
    • 1
  • Lei Chen
    • 1
  • Yiyun Zhang
    • 1
  • Xiangyang Kang
    • 1
  • Zhiyi Zhang
    • 1
  • Yanwei Wang
    • 1
  1. 1.National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and BiotechnologyBeijing Forestry UniversityBeijingPeople’s Republic of China