Functional & Integrative Genomics

, 11:407

Identification of haptoglobin and apolipoprotein A-I as biomarkers for high altitude pulmonary edema

  • Yasmin Ahmad
  • Dhananjay Shukla
  • Iti Garg
  • Narendra K. Sharma
  • Saurabh Saxena
  • V. K. Malhotra
  • Kalpana Bhargava
Original Paper

DOI: 10.1007/s10142-011-0234-3

Cite this article as:
Ahmad, Y., Shukla, D., Garg, I. et al. Funct Integr Genomics (2011) 11: 407. doi:10.1007/s10142-011-0234-3

Abstract

We have investigated the plasma proteome using 2D gel electrophoresis and matrix-assisted laser desorption/ionization tandem time of flight from patients with high altitude pulmonary edema (HAPE). A complete proteomic analysis was performed on 20 patients with HAPE and ten healthy sea level controls. In total, we have identified 25 protein spots in human plasma and found that 14 of them showed altered changes in HAPE patients, which mainly were acute phase proteins (APPs), compliment components, and apolipoproteins among others. Among the APPs, haptoglobin α2 chain, haptoglobin β chain, transthyretin, and plasma retinol binding precursor showed overexpression in HAPE patients as compared to controls. To validate the result of proteomic analysis, two proteins were selected for enzyme-linked immunosorbent assay and Western blotting analysis. Our data conclusively shows that two proteins, haptoglobin and apolipoprotein A-I are upregulated in plasma of HAPE patients. These proteins may provide a fast and effective control of inflammatory damage until the subsequent mechanisms can begin to operate. Taken together, our findings further support the hypothesis that inflammatory response system is linked to the pathophysiology of HAPE.

Keywords

High-altitude pulmonary edemaHuman blood plasmaTwo-dimensional gel electrophoresis (2DE)Mass fingerprintingAcute phase proteins

Copyright information

© Springer-Verlag 2011

Authors and Affiliations

  • Yasmin Ahmad
    • 1
  • Dhananjay Shukla
    • 2
  • Iti Garg
    • 3
  • Narendra K. Sharma
    • 1
  • Saurabh Saxena
    • 2
  • V. K. Malhotra
    • 2
  • Kalpana Bhargava
    • 1
  1. 1.Peptide and Proteomics Division, Defence Institute of Physiology & Allied Sciences (DIPAS), Defence Research & Development Organization (DRDO)Ministry of DefenceDelhiIndia
  2. 2.Experimental Biology Division, Defence Institute of Physiology & Allied Sciences (DIPAS), Defence Research & Development Organization (DRDO)Ministry of DefenceDelhiIndia
  3. 3.Department of Hematology, Defence Institute of Physiology & Allied Sciences (DIPAS), Defence Research & Development Organization (DRDO)Ministry of DefenceDelhiIndia