Functional & Integrative Genomics

, Volume 11, Issue 1, pp 193–202

Homeorhetic adaptation to lactation: comparative transcriptome analysis of mammary, liver, and adipose tissue during the transition from pregnancy to lactation in rats

  • Osman V. Patel
  • Theresa Casey
  • Heather Dover
  • Karen Plaut
Short Communication

DOI: 10.1007/s10142-010-0193-0

Cite this article as:
Patel, O.V., Casey, T., Dover, H. et al. Funct Integr Genomics (2011) 11: 193. doi:10.1007/s10142-010-0193-0

Abstract

Tissue-specific shifts in a dam’s metabolism to support fetal and neonatal growth during pregnancy and lactation are controlled by differential expression of regulatory genes. The goal of this study was to identify a more detailed cohort of genes in mammary, liver, and adipose tissue that are transcriptionally controlled during the pregnancy to lactation evolution and explore the relationship of these genes to core clock genes. Total RNA was isolated from mammary, liver and adipose tissues collected from rat dams on day 20 of pregnancy (P20) and day 1 of lactation (L1) and gene expression was measured using Rat 230 2.0 Affymetrix GeneChips. Gene functional analysis revealed that pathway associated metabolism (carbohydrate, amino acid, lipid, cholesterol, protein) were enriched (P < 0.001) in the mammary gland during P20 to L1 transition. Approximately 50% of the genes associated with solute transport, as well as lipogenesis were up-regulated in the mammary gland during P20 to L1 transition compared to 10% in liver and 15% in adipose tissue. Genes engaged in conveying glucose (INSR, GLUT1, GLUT4, SGLT1, and SGLT2), bicarbonate (SLC4), sodium (SLC9), zinc (SLC30), copper (SLC31), iron (SLC40) in tandem with rate-limiting lipogenic genes (ACACA, FASN, PRLR, SREBP2, THRSP) were specifically enriched in the mammary gland during the P20 to L1 evolution. Our results provide insight into a cross-tissue transcriptional repertoire that is associated with homeorhetic adaptation needed to support lactation, and at the onset of lactation the mammary gland becomes a factory for macromolecular biosynthesis through inducing genes participating in nutrient transfer and lipid biosynthesis.

Keywords

Mammary Liver Adipose Pregnancy Lactation Microarray 

Supplementary material

10142_2010_193_MOESM1_ESM.xls (66 kb)
Supplementary Table 1(XLS 66 kb)
10142_2010_193_MOESM2_ESM.doc (59 kb)
Supplementary Table 2The numerical fold-change and P value for genes associated with solute transport, TCA cycle and fatty acid metabolism across adipose, liver and mammary tissues during the pregnancy to lactation transition. (DOC 59 kb)

Copyright information

© Springer-Verlag 2010

Authors and Affiliations

  • Osman V. Patel
    • 1
    • 2
  • Theresa Casey
    • 2
    • 3
  • Heather Dover
    • 2
  • Karen Plaut
    • 2
  1. 1.Department of Cell and Molecular BiologyGrand Valley State UniversityAllendaleUSA
  2. 2.Department of Animal ScienceMichigan State UniversityEast LansingUSA
  3. 3.Department of Animal SciencePurdue UniversityWest LafayetteUSA

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