Functional & Integrative Genomics

, Volume 8, Issue 4, pp 421–427

Components of the gene network associated with genotype-dependent response of wheat to the Fusarium mycotoxin deoxynivalenol

  • Stephanie Walter
  • Josephine M. Brennan
  • Chanemougasoundharam Arunachalam
  • Khairul I. Ansari
  • Xuejun Hu
  • Mojibur R. Khan
  • Friederike Trognitz
  • Bodo Trognitz
  • Gerald Leonard
  • Damian Egan
  • Fiona M. Doohan
Short Communication

DOI: 10.1007/s10142-008-0089-4

Cite this article as:
Walter, S., Brennan, J.M., Arunachalam, C. et al. Funct Integr Genomics (2008) 8: 421. doi:10.1007/s10142-008-0089-4

Abstract

The Fusarium mycotoxin deoxynivalenol (DON) facilitates fungal spread within wheat tissue and the development of Fusarium head blight disease. The ability of wheat spikelets to resist DON-induced bleaching is genotype-dependent. In wheat cultivar (cv.) CM82036 DON resistance is associated with a quantitative trait locus, Fhb1, located on the short arm of chromosome 3B. Gene expression profiling (microarray and real-time RT-PCR analyses) of DON-treated spikelets of progeny derived from a cross between cv. CM82036 and the DON-susceptible cv. Remus discriminated ten toxin-responsive transcripts associated with the inheritance of DON resistance and Fhb1. These genes do not exclusively map to Fhb1. Based on the putative function of the ten Fhb1-associated transcripts, we discuss how cascades involving classical metabolite biotransformation and sequestration processes, alleviation of oxidative stress and promotion of cell survival might contribute to the host response and defence against DON.

Keywords

Fhb1TrichothecenesWheat cDNA microarray

Supplementary material

10142_2008_89_MOESM1_ESM.doc (121 kb)
ESM Materials and methods(DOC 121 KB)
10142_2008_89_MOESM2_ESM.doc (53 kb)
ESM Results(DOC 53 KB)
10142_2008_89_MOESM3_ESM.xls (2.8 mb)
ESM Table S1(XLS 2.80 MB)
10142_2008_89_MOESM4_ESM.doc (38 kb)
ESM Table S2(DOC 38 KB)
10142_2008_89_MOESM5_ESM.doc (48 kb)
ESM Table S3(DOC 48.5 KB)
10142_2008_89_MOESM6_ESM.xls (48 kb)
ESM Table S4(XLS 48.5 KB)
10142_2008_89_MOESM7_ESM.doc (42 kb)
ESM Table S5(DOC 42.5 KB)
10142_2008_89_MOESM8_ESM.pdf (226 kb)
ESM Fig. S1(PDF 226 KB)
10142_2008_89_MOESM9_ESM.pdf (73 kb)
ESM Fig. S2(PDF 73.3 KB)
10142_2008_89_MOESM10_ESM.pdf (77 kb)
ESM Fig. S3(PDF 77.3.2 KB)

Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Stephanie Walter
    • 1
    • 6
  • Josephine M. Brennan
    • 1
    • 4
  • Chanemougasoundharam Arunachalam
    • 1
  • Khairul I. Ansari
    • 1
    • 3
  • Xuejun Hu
    • 1
    • 5
  • Mojibur R. Khan
    • 1
  • Friederike Trognitz
    • 2
  • Bodo Trognitz
    • 2
  • Gerald Leonard
    • 1
  • Damian Egan
    • 1
  • Fiona M. Doohan
    • 1
  1. 1.Molecular Plant-Microbe Interactions Laboratory, School of Biology and Environmental Sciences, College of Life SciencesUniversity College DublinBelfieldIreland
  2. 2.Biogenetics-Natural Resources Division, Bioresources DepartmentAustrian Research Centers GmbH (ARC)ViennaAustria
  3. 3.Department of Chemistry and BiochemistryUniversity of Texas at ArlingtonArlingtonUSA
  4. 4.Plant Health LaboratoryDepartment of Agriculture and FoodBackwestonIreland
  5. 5.Medical CollegeDalian UniversityDalianChina
  6. 6.Department of Integrated Pest Management, Research Centre FlakkebjergUniversity of AarhusForsøgsvej 1Denmark