Functional & Integrative Genomics

, Volume 7, Issue 4, pp 317–333

Transcript abundance profiles reveal larger and more complex responses of grapevine to chilling compared to osmotic and salinity stress

Authors

  • Elizabeth A. R. Tattersall
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
  • Jérôme Grimplet
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
  • Laurent DeLuc
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
  • Matthew D. Wheatley
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
  • Delphine Vincent
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
  • Craig Osborne
    • Department of Animal BiotechnologyUniversity of Nevada
  • Ali Ergül
    • Biotechnology InstituteAnkara University
  • Evan Lomen
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
  • Robert R. Blank
    • USDA-Agricultural Research Service
  • Karen A. Schlauch
    • Boston University School of Medicine, Department of Genetics and GenomicsBoston University
  • John C. Cushman
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
    • Department of Biochemistry & Molecular BiologyUniversity of Nevada
Original Paper

DOI: 10.1007/s10142-007-0051-x

Cite this article as:
Tattersall, E.A.R., Grimplet, J., DeLuc, L. et al. Funct Integr Genomics (2007) 7: 317. doi:10.1007/s10142-007-0051-x

Abstract

Cabernet Sauvignon grapevines were exposed to sudden chilling (5°C), water deficit (PEG), and an iso-osmotic salinity (120 mM NaCl and 12 mM CaCl2) for 1, 4, 8, and 24 h. Stomatal conductance and stem water potentials were significantly reduced after stress application. Microarray analysis of transcript abundance in shoot tips detected no significant differences in transcript abundance between salinity and PEG before 24 h. Chilling stress relates to changes in membrane structure, and transcript abundance patterns were predicted to reflect this. Forty-three percent of transcripts affected by stress vs control for 1 through 8 h were affected only by chilling. The functional categories most affected by stress included metabolism, protein metabolism, and signal transduction. Osmotic stress affected more protein synthesis and cell cycle transcripts, whereas chilling affected more calcium signaling transcripts, indicating that chilling has more complex calcium signaling. Stress affected many hormone (ABA, ethylene, and jasmonate) and transcription factor transcripts. The concentrations and transporter transcripts of several anions increased with time, including nitrate, sulfate, and phosphate. The transcript abundance changes in this short-term study were largely the same as a gradually applied long-term salinity and water-deficit study (Cramer et al. Funct Integr Genomics 7:111–134, 2007), but the reverse was not true, indicating a larger and more complex response in the acclimation process of a gradual long-term stress.

Keywords

Vitis viniferaAbiotic stressMicroarray

Supplementary material

Copyright information

© Springer-Verlag 2007