Original Paper

Functional & Integrative Genomics

, Volume 2, Issue 6, pp 282-291

First online:

Monitoring the expression pattern of around 7,000 Arabidopsis genes under ABA treatments using a full-length cDNA microarray

  • Motoaki SekiAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
  • , Junko IshidaAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
  • , Mari NarusakaAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
  • , Miki FujitaAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
  • , Tokihiko NanjoAffiliated withLaboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3–1-1 Koyadai, Tsukuba 305-0074, Japan
  • , Taishi UmezawaAffiliated withLaboratory of Plant Molecular Biology, RIKEN Tsukuba Institute, 3–1-1 Koyadai, Tsukuba 305-0074, Japan
  • , Asako KamiyaAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
  • , Maiko NakajimaAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
  • , Akiko EnjuAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
    • , Tetsuya SakuraiAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
    • , Masakazu SatouAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
    • , Kenji AkiyamaAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan
    • , Kazuko Yamaguchi-ShinozakiAffiliated withBiological Resources Division, Japan International Research Center for Agricultural Sciences, Ministry of Agriculture, Forestry, and Fisheries, 2–1 Ohwashi, Tsukuba, Ibaraki 305–0851, Japan
    • , Piero CarninciAffiliated withGenome Science Laboratory, RIKEN Tsukuba Institute, 3–1-1 Koyadai, Tsukuba 305-0074, Japan
    • , Jun KawaiAffiliated withGenome Science Laboratory, RIKEN Tsukuba Institute, 3–1-1 Koyadai, Tsukuba 305-0074, Japan
    • , Yoshihide HayashizakiAffiliated withGenome Science Laboratory, RIKEN Tsukuba Institute, 3–1-1 Koyadai, Tsukuba 305-0074, Japan
    • , Kazuo ShinozakiAffiliated withPlant Mutation Exploration Team, Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center, 3–1-1 Koyadai, Tsukuba 305–0074, Japan

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Abstract.

Full-length cDNAs are essential for functional analysis of plant genes. Recently, cDNA microarray analysis has been developed for quantitative analysis of global and simultaneous analysis of expression profiles. Microarray technology is a powerful tool for identifying genes induced by environmental stimuli or stress and for analyzing their expression profiles in response to environmental signals. We prepared an Arabidopsis full-length cDNA microarray containing around 7,000 independent full-length cDNA groups and analyzed the expression profiles of genes. The transcripts of 245, 54, 299 and 213 genes increased after abscisic acid (ABA), drought-, cold-, and salt-stress treatments, respectively, with inducibilities more than fivefold compared with those of control genes. The cDNA microarray analysis showed that many ABA-inducible genes were induced after drought- and high-salinity-stress treatments, and that there is more crosstalk between drought and ABA responses than between ABA and cold responses. Among the ABA-inducible genes identified, we identified 22 transcription factor genes, suggesting that many transcriptional regulatory mechanisms exist in the ABA signal transduction pathways. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s10142-002-0070-6 or from http://www.gsc.riken.go.jp/Plant/index.html.

ABA cDNA microarray Arabidopsis thaliana Full-length cDNA