, Volume 3, Issue 3, pp 275-280

Polymorphisms of GA/GT Microsatellite Loci from Anguilla japonica

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Abstract:

Six novel microsatellite loci, containing (GA)15\N17 or (GT)10\N19 perfect tandem repeats, were isolated and characterized for the catadromous eel Anguilla japonica. The allelic size of the 6 loci ranged from 79 to 226 bp in length. All loci were polymorphic with a mean number of 14.7 alleles per locus and a mean heterozygosity of 0.67, suggesting higher polymorphism than that of freshwater and anadromous fishes, but lower than that of marine fishes. Genotype diversity of the 6 loci ranged from 0.22 to 0.61 with a mean value of approximately 0.5. Cross-species amplification showed that 5 of the 6 microsatellite primers proved to be useful in addressing questions of population genetics for all Anguilla species.

Received October 11, 2000; accepted December 29, 2000.