Polymorphisms of GA/GT Microsatellite Loci from Anguilla japonica
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- Tseng, M., Chen, C., Kao, H. et al. Mar. Biotechnol. (2001) 3: 275. doi:10.1007/s101260000076
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Six novel microsatellite loci, containing (GA)15\N17 or (GT)10\N19 perfect tandem repeats, were isolated and characterized for the catadromous eel Anguilla japonica. The allelic size of the 6 loci ranged from 79 to 226 bp in length. All loci were polymorphic with a mean number of 14.7 alleles per locus and a mean heterozygosity of 0.67, suggesting higher polymorphism than that of freshwater and anadromous fishes, but lower than that of marine fishes. Genotype diversity of the 6 loci ranged from 0.22 to 0.61 with a mean value of approximately 0.5. Cross-species amplification showed that 5 of the 6 microsatellite primers proved to be useful in addressing questions of population genetics for all Anguilla species.