Characterization and Quantification of Chitosan Extracted from Nacre of the Abalone Haliotis tuberculata and the Oyster Pinctada maxima
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This study was performed to characterize and quantify chitosan by simple physicochemical methods (infrared spectroscopy and potentiometric measurements). These procedures were validated with well-characterized chitosan before being used to investigate chitosan in nacre of the abalone Haliotis tuberculata and of the giant oyster Pinctada maxima. Potentiometric study revealed a chitosan extract from the nacre of H. tuberculata with a degree of deacetylation of around 88% and an intrinsic pK of 6.5. According to infrared and potentiometric data, a low yield (η) of extraction was calculated (η= 0.064%). For experiments performed on the nacre of P. maxima, and in spite of more stringent deacetylation conditions, results suggested that a chitin-protein complex (η= 0.053%) was isolated rather than chitosan.
- Characterization and Quantification of Chitosan Extracted from Nacre of the Abalone Haliotis tuberculata and the Oyster Pinctada maxima
Volume 3, Issue 1 , pp 36-44
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- Key words: nacre, chitosan, Haliotis tuberculata, Pinctada maxima.
- Author Affiliations
- A1. F.R.E. 2125, CNRS-UBO-MNHN, Chimie et Biologie des Substances Naturelles, 6 rue de l'Université, 29 000 Quimper, France, FR
- A2. U.M.R. 8572, CNRS-MNHN, Laboratoire de Physiologie Générale et Comparée, 7 rue Cuvier, 75 231 Paris Cedex 05, France, FR