Marine Biotechnology

, Volume 15, Issue 3, pp 340–348

Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

Authors

  • Satoru Kurokawa
    • Animal Health Department of Research and Development Agricultural and Veterinary DivisionMeiji Seika Pharma
  • Jun Kabayama
    • Animal Health Department of Research and Development Agricultural and Veterinary DivisionMeiji Seika Pharma
  • Tsuguaki Fukuyasu
    • Animal Health Department of Research and Development Agricultural and Veterinary DivisionMeiji Seika Pharma
  • Seong Don Hwang
    • Institute of Marine Industry, Department of Marine Biology and Aquaculture, College of Marine ScienceGyeongsang National University
  • Chan-Il Park
    • Institute of Marine Industry, Department of Marine Biology and Aquaculture, College of Marine ScienceGyeongsang National University
  • Seong-Bin Park
    • Aquatic Biotechnology Center of WCU Project, College of Veterinary MedicineGyeongsang National University
  • Carmelo S. del Castillo
    • Aquatic Biotechnology Center of WCU Project, College of Veterinary MedicineGyeongsang National University
  • Jun-ichi Hikima
    • Aquatic Biotechnology Center of WCU Project, College of Veterinary MedicineGyeongsang National University
  • Tae-Sung Jung
    • Aquatic Biotechnology Center of WCU Project, College of Veterinary MedicineGyeongsang National University
  • Hidehiro Kondo
    • Laboratory of Genome ScienceTokyo University of Marine Science and Technology
  • Ikuo Hirono
    • Laboratory of Genome ScienceTokyo University of Marine Science and Technology
  • Haruko Takeyama
    • Department of Life Science and Medical BioscienceWaseda University
    • Aquatic Biotechnology Center of WCU Project, College of Veterinary MedicineGyeongsang National University
    • Consolidated Research Institute for Advanced Science and Medical CareWaseda University
Original Article

DOI: 10.1007/s10126-012-9492-x

Cite this article as:
Kurokawa, S., Kabayama, J., Fukuyasu, T. et al. Mar Biotechnol (2013) 15: 340. doi:10.1007/s10126-012-9492-x

Abstract

Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.

Keywords

Mycobacterium marinum Mycobacterium sp. Phylogenetic analysis MALDI Biotyper

Copyright information

© Springer Science+Business Media New York 2012