Original Article

Marine Biotechnology

, Volume 15, Issue 3, pp 340-348

Bacterial Classification of Fish-Pathogenic Mycobacterium Species by Multigene Phylogenetic Analyses and MALDI Biotyper Identification System

  • Satoru KurokawaAffiliated withAnimal Health Department of Research and Development Agricultural and Veterinary Division, Meiji Seika Pharma
  • , Jun KabayamaAffiliated withAnimal Health Department of Research and Development Agricultural and Veterinary Division, Meiji Seika Pharma
  • , Tsuguaki FukuyasuAffiliated withAnimal Health Department of Research and Development Agricultural and Veterinary Division, Meiji Seika Pharma
  • , Seong Don HwangAffiliated withInstitute of Marine Industry, Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University
  • , Chan-Il ParkAffiliated withInstitute of Marine Industry, Department of Marine Biology and Aquaculture, College of Marine Science, Gyeongsang National University
  • , Seong-Bin ParkAffiliated withAquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University
  • , Carmelo S. del CastilloAffiliated withAquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University
  • , Jun-ichi HikimaAffiliated withAquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University
  • , Tae-Sung JungAffiliated withAquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National University
    • , Hidehiro KondoAffiliated withLaboratory of Genome Science, Tokyo University of Marine Science and Technology
    • , Ikuo HironoAffiliated withLaboratory of Genome Science, Tokyo University of Marine Science and Technology
    • , Haruko TakeyamaAffiliated withDepartment of Life Science and Medical Bioscience, Waseda University
    • , Takashi AokiAffiliated withAquatic Biotechnology Center of WCU Project, College of Veterinary Medicine, Gyeongsang National UniversityConsolidated Research Institute for Advanced Science and Medical Care, Waseda University Email author 

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Abstract

Mycobacterium marinum is difficult to distinguish from other species of Mycobacterium isolated from fish using biochemical methods. Here, we used genetic and proteomic analyses to distinguish three Mycobacterium strains: M. marinum strains MB2 and Europe were isolated from tropical and marine fish in Thailand and Europe, and Mycobacterium sp. 012931 strain was isolated from yellowtail in Japan. In phylogenetic trees based on gyrB, rpoB, and Ag85B genes, Mycobacterium sp. 012931 clustered with M. marinum strains MB2 and Europe, but in trees based on 16S rRNA, hsp65, and Ag85A genes Mycobacterium sp. 012931 did not cluster with the other strains. In proteomic analyses using a Bruker matrix-assisted laser desorption ionization Biotyper, the mass profile of Mycobacterium sp. 012931 differed from the mass profiles of the other two fish M. marinum strains. Therefore, Mycobacterium sp. 012931 is similar to M. marinum but is not the same, suggesting that it could be a subspecies of M. marinum.

Keywords

Mycobacterium marinum Mycobacterium sp. Phylogenetic analysis MALDI Biotyper