Marine Biotechnology

, Volume 14, Issue 2, pp 237–244

A Microsatellite Linkage Map of Striped Bass (Morone saxatilis) Reveals Conserved Synteny with the Three-Spined Stickleback (Gasterosteus aculeatus)

  • Sixin Liu
  • Caird E. RexroadIII
  • Charlene R. Couch
  • Jan F. Cordes
  • Kimberly S. Reece
  • Craig V. Sullivan
Original Article

DOI: 10.1007/s10126-011-9407-2

Cite this article as:
Liu, S., Rexroad, C.E., Couch, C.R. et al. Mar Biotechnol (2012) 14: 237. doi:10.1007/s10126-011-9407-2

Abstract

The striped bass (Morone saxatilis) and its relatives (genus Morone) are of great importance to fisheries and aquaculture in North America. As part of a collaborative effort to employ molecular genetics technologies in striped bass breeding programs, we previously developed nearly 500 microsatellite markers. The objectives of this study were to construct a microsatellite linkage map of striped bass and to examine conserved synteny between striped bass and three-spined stickleback (Gasterosteus aculeatus). Of 480 microsatellite markers screened for polymorphism, 289 informative markers were identified and used to genotype two half-sib mapping families. Twenty-six linkage groups were assembled, and only two markers remain unlinked. The sex-averaged map spans 1,623.8 cM with an average marker density of 5.78 cM per marker. Among 287 striped bass microsatellite markers assigned to linkage groups, 169 (58.9%) showed homology to sequences on stickleback chromosomes or scaffolds. Comparison between the stickleback genome and the striped bass linkage map revealed conserved synteny between these two species. This is the first linkage map for any of the Morone species. This map will be useful for molecular mapping and marker-assisted selection of genes of interest in striped bass breeding programs. The conserved synteny between striped bass and stickleback will facilitate fine mapping of genome regions of interest and will serve as a new resource for comparative mapping with other Perciform fishes such as European sea bass (Dicentrarchus labrax), gilthead sea bream (Sparus aurata), and tilapia (Oreochromis ssp.).

Keywords

Striped bass Microsatellite Linkage map Synteny Three-spined stickleback 

Supplementary material

10126_2011_9407_MOESM1_ESM.doc (712 kb)
Additional file 1Striped bass microsatellite markers screened for polymorphism (DOC 712 kb)
10126_2011_9407_MOESM2_ESM.xls (38 kb)
Additional file 2Numbers of informative meiosis of microsatellite markers (XLS 38 kb)
10126_2011_9407_MOESM3_ESM.pdf (36 kb)
Additional file 3Female linkage map of striped bass. The map distances are in Kosambi cM. The markers are color-coded based on LOD scores: black LOD ≥ 3, red LOD ≥ 2, green LOD ≥ 1, and pink LOD ≥ 0 (PDF 35 kb)
10126_2011_9407_MOESM4_ESM.pdf (37 kb)
Additional file 4Male linkage map of striped bass. The map distances are in Kosambi cM. The markers are color-coded based on LOD scores: black LOD ≥ 3, red LOD ≥ 2, green LOD ≥ 1, and pink LOD ≥ 0 (PDF 37 kb)
10126_2011_9407_MOESM5_ESM.ppt (888 kb)
Additional file 5Comparative maps between striped bass linkage map (Kosambi cM) and chromosomes of three-spined stickleback (100 kb). The markers are color-coded based on LOD scores: black LOD ≥ 3, red LOD ≥ 2, green LOD ≥ 1, and pink LOD ≥ 0 (PPT 888 kb)
10126_2011_9407_MOESM6_ESM.xls (48 kb)
Additional file 6The best hits of European sea bass BAC-end sequences or whole genome shotgun sequences using the masked sequences of striped bass microsatellite markers as queries for BLASTn searches (XLS 47 kb)

Copyright information

© Springer Science+Business Media, LLC (outside the USA)  2011

Authors and Affiliations

  • Sixin Liu
    • 1
  • Caird E. RexroadIII
    • 1
  • Charlene R. Couch
    • 2
  • Jan F. Cordes
    • 3
  • Kimberly S. Reece
    • 3
  • Craig V. Sullivan
    • 4
  1. 1.USDA/ARS National Center of Cool and Cold Water AquacultureKearneysvilleUSA
  2. 2.Department of GeneticsNorth Carolina State UniversityRaleighUSA
  3. 3.Department of Environmental and Aquatic Animal HealthVirginia Institute of Marine ScienceGloucester PointUSA
  4. 4.Department of BiologyNorth Carolina State UniversityRaleighUSA

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