Marine Biotechnology

, Volume 12, Issue 4, pp 430–438

Isolation and Characterisation of a High-Efficiency Desaturase and Elongases from Microalgae for Transgenic LC-PUFA Production

  • James R. Petrie
  • Qing Liu
  • Anne M. Mackenzie
  • Pushkar Shrestha
  • Maged P. Mansour
  • Stan S. Robert
  • Dion F. Frampton
  • Susan I. Blackburn
  • Peter D. Nichols
  • Surinder P. Singh
Original Article

DOI: 10.1007/s10126-009-9230-1

Cite this article as:
Petrie, J.R., Liu, Q., Mackenzie, A.M. et al. Mar Biotechnol (2010) 12: 430. doi:10.1007/s10126-009-9230-1

Abstract

The production of long-chain polyunsaturated fatty acids from precursor molecules linoleic acid (LA; 18:2ω6) and α-linolenic acid (ALA; 18:3ω3) is catalysed by sequential desaturase and elongase reactions. We report the isolation of a front-end Δ6-desaturase gene from the microalgae Ostreococcus lucimarinus and two elongase genes, a Δ6-elongase and a Δ5-elongase, from the microalga Pyramimonas cordata. These enzymes efficiently convert their respective substrates when transformed in yeast (39–75% conversion for ω3 substrate fatty acids), and the Δ5-elongase in particular displays higher elongation efficiency (75% for conversion of eicosapentaenoic acid (20:5ω3) to docosapentaenoic acid (22:5ω3)) than previously reported genes. In addition, the Δ6-desaturase is homologous with acyl-CoA desaturases and shows a strong preference for the ω3 substrate ALA.

Keywords

n-3 LC-PUFA Ostreococcus lucimarinus Pyramimonas cordata Δ6-Desaturase Δ6-Elongase Δ5-Elongase 

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • James R. Petrie
    • 1
  • Qing Liu
    • 1
  • Anne M. Mackenzie
    • 1
  • Pushkar Shrestha
    • 1
  • Maged P. Mansour
    • 2
  • Stan S. Robert
    • 2
  • Dion F. Frampton
    • 2
  • Susan I. Blackburn
    • 2
  • Peter D. Nichols
    • 2
  • Surinder P. Singh
    • 1
  1. 1.Food Futures National Research FlagshipCSIRO Plant IndustryCanberraAustralia
  2. 2.Food Futures National Research FlagshipCSIRO Marine and Atmospheric ResearchHobartAustralia

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