Marine Biotechnology

, Volume 12, Issue 3, pp 326–339

Microarray-Based Identification of Gonad Transcripts Differentially Expressed Between Lines of Pacific Oyster Selected to Be Resistant or Susceptible to Summer Mortality

Authors

  • Elodie Fleury
    • UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”
  • Jeanne Moal
    • UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”
  • Viviane Boulo
    • Ifremer, CNRS Université de Montpellier 2
  • Jean-Yves Daniel
    • UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”
  • David Mazurais
    • Ifremer, UMR 1067 Nutrition, aquaculture, et génomique
  • Alain Hénaut
    • CNRS Université Pierre et Marie Curie
  • Charlotte Corporeau
    • UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”
  • Pierre Boudry
    • UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”
  • Pascal Favrel
    • UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”
    • UMR M100 Ifremer- Université de Caen Basse Normandie “Physiologie et Ecophysiologie des Mollusques Marins”
Original Article

DOI: 10.1007/s10126-009-9227-9

Cite this article as:
Fleury, E., Moal, J., Boulo, V. et al. Mar Biotechnol (2010) 12: 326. doi:10.1007/s10126-009-9227-9

Abstract

Summer mortality of the Pacific oyster Crassostrea gigas is the result of a complex interaction between oysters, their environment, and pathogens. Heredity appears to be a major factor determining the sensitivity of oysters to summer mortality, allowing resistant (R) and susceptible (S) lines to be produced. We conducted genome-wide expression profiling of R and S gonads during the 3-month period preceding a summer mortality event, using a cDNA microarray that we designed. ANOVA analysis revealed that 34 genes were differentially expressed between R and S lines on four dates preceding the mortality event. Annotation of some of these genes highlights reproduction and its allocation and antioxidant defenses as the main pathways that operate differentially between R and S lines. This transcriptional analysis provides new indications to define markers for quantitative trait loci searches and functional studies and evaluate the potential role of each gene in the resistance to summer mortality.

Keywords

MolluscaBivalviaGene expressioncDNA microarraysReproductionOxidative stressEnergy metabolism

Copyright information

© Springer Science+Business Media, LLC 2009