Marine Biotechnology

, Volume 11, Issue 3, pp 419–429

Development of a Whole-organism Model to Screen New Compounds for Sun Protection

  • Yun-Hsin Wang
  • Chi-Chung Wen
  • Zhi-Shiang Yang
  • Chien-Chung Cheng
  • Jen-Ning Tsai
  • Chia-Chen Ku
  • Hsin-Ju Wu
  • Yau-Hung Chen
Original Article

DOI: 10.1007/s10126-008-9159-9

Cite this article as:
Wang, Y., Wen, C., Yang, Z. et al. Mar Biotechnol (2009) 11: 419. doi:10.1007/s10126-008-9159-9

Abstract

We used zebrafish as a whole-organism model to screen new compounds for sun protection activity. First of all, we designed a series of UVB exposure experiments and recorded the phenotypic changes of zebrafish embryos. Results showed that 100 mJ/cm2 of UVB given six times separated by 30 min intervals is the best condition. Fin malformation (reduced and/or absent fin) phenotypes are the most evident consequences after exposure to UVB. Each fin was affected by UVB, including pelvic, ventral, caudal, and dorsal fin, but pelvic fin seemed to be the most sensitive target after UVB exposure. We furthermore carried out “prevention” and “treatment” experiments using green tea extract and/or (−)-epigallocatechin (EGCG) to test this whole-organism model by observing the morphological changes of all fins (especially pelvic fin) after UVB exposure. Effects of UVB, green tea extract and EGCG on fin development were assessed using the Kaplan–Meier analysis, log-rank test and Cox proportional hazards regression. Results showed that a zebrafish pelvic fin in the UVB + green tea (treatment) group is 5.51 (range from 2.39 to 14.90) times, one in the UVB + green tea (prevention) group is 7.04 (range from 3.11 to 18.92) times, and one in the 25 ppm of EGCG (prevention) group is 22.19 (range from 9.40 to 61.50) times more likely to return to normal fin than one in the UVB only group. On the basis of these observations, we believe this model is effective for screening the higher stability and lower toxicity of new compounds, such as small chemicals which are derivative from EGCG or other dietary agents for sun protection.

Keywords

EGCGFinTeaUVZebrafish

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Yun-Hsin Wang
    • 1
  • Chi-Chung Wen
    • 2
  • Zhi-Shiang Yang
    • 3
  • Chien-Chung Cheng
    • 4
  • Jen-Ning Tsai
    • 5
  • Chia-Chen Ku
    • 6
  • Hsin-Ju Wu
    • 3
  • Yau-Hung Chen
    • 3
    • 7
  1. 1.Department of ResearchSun Yat-Sen Cancer CenterTaipeiTaiwan
  2. 2.Department of MathematicsTamkang UniversityTamsuiTaiwan
  3. 3.Graduate Institute of Life SciencesTamkang UniversityTamsuiTaiwan
  4. 4.Department of Applied ChemistryNational Chia-Yi UniversityChia-YiTaiwan
  5. 5.School of Medical Laboratory and BiotechnologyChung Shan Medical UniversityTaichungTaiwan
  6. 6.Department of ChemistryTamkang UniversityTamsuiTaiwan
  7. 7.Life Sciences Development CenterTamkang UniversityTamsuiTaiwan