Marine Biotechnology

, Volume 11, Issue 3, pp 410–418

Isolation and Characterisation of a Δ5-fatty Acid Elongase from the Marine Microalga Pavlova salina

Authors

  • Stanley S. Robert
    • Food Futures National Research FlagshipCSIRO Marine and Atmospheric Research
  • James R. Petrie
    • Food Futures National Research FlagshipCSIRO Plant Industry
  • Xue-Rong Zhou
    • Food Futures National Research FlagshipCSIRO Plant Industry
  • Maged P. Mansour
    • Food Futures National Research FlagshipCSIRO Marine and Atmospheric Research
  • Susan I. Blackburn
    • Food Futures National Research FlagshipCSIRO Marine and Atmospheric Research
  • Allan G. Green
    • CSIRO Plant Industry
  • Surinder P. Singh
    • Food Futures National Research FlagshipCSIRO Plant Industry
    • Food Futures National Research FlagshipCSIRO Marine and Atmospheric Research
Original Article

DOI: 10.1007/s10126-008-9157-y

Cite this article as:
Robert, S.S., Petrie, J.R., Zhou, X. et al. Mar Biotechnol (2009) 11: 410. doi:10.1007/s10126-008-9157-y

Abstract

The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). A full-length cDNA sequence, designated PsElo5, was isolated from P. salina. Sequence alignment showed that the gene was homologous to corresponding ELO-type elongases from other microalgae. Heterologous expression of PsElo5 in yeast and in higher plants confirmed that it encodes a specific Δ5-elongase activity as predicted and, furthermore, within the n-3 pathway, the elongation activity was confined exclusively to EPA.

Keywords

n-3 LC-PUFAΔ5 elongasePavlova salinaYeast modelArabidopsis model

Copyright information

© Springer Science+Business Media, LLC 2008