Original Article

Marine Biotechnology

, Volume 11, Issue 3, pp 410-418

Isolation and Characterisation of a Δ5-fatty Acid Elongase from the Marine Microalga Pavlova salina

  • Stanley S. RobertAffiliated withFood Futures National Research Flagship, CSIRO Marine and Atmospheric Research
  • , James R. PetrieAffiliated withFood Futures National Research Flagship, CSIRO Plant Industry
  • , Xue-Rong ZhouAffiliated withFood Futures National Research Flagship, CSIRO Plant Industry
  • , Maged P. MansourAffiliated withFood Futures National Research Flagship, CSIRO Marine and Atmospheric Research
  • , Susan I. BlackburnAffiliated withFood Futures National Research Flagship, CSIRO Marine and Atmospheric Research
  • , Allan G. GreenAffiliated withCSIRO Plant Industry
  • , Surinder P. SinghAffiliated withFood Futures National Research Flagship, CSIRO Plant Industry
  • , Peter D. NicholsAffiliated withFood Futures National Research Flagship, CSIRO Marine and Atmospheric Research Email author 

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Abstract

The marine microalga Pavlova salina (Haptophyta, Pavlovophyceae) produces lipids containing approximately 50% n-3 long-chain polyunsaturated fatty acids including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). A full-length cDNA sequence, designated PsElo5, was isolated from P. salina. Sequence alignment showed that the gene was homologous to corresponding ELO-type elongases from other microalgae. Heterologous expression of PsElo5 in yeast and in higher plants confirmed that it encodes a specific Δ5-elongase activity as predicted and, furthermore, within the n-3 pathway, the elongation activity was confined exclusively to EPA.

Keywords

n-3 LC-PUFA Δ5 elongase Pavlova salina Yeast model Arabidopsis model