Marine Biotechnology

, 11:74

Repair of UVB-Damaged Skin by the Antioxidant Sulphated Flavone Glycoside Thalassiolin B Isolated from the Marine Plant Thalassia testudinum Banks ex König

  • Erik L. Regalado
  • María Rodríguez
  • Roberto Menéndez
  • Ángel A. Concepción
  • Clara Nogueiras
  • Abilio Laguna
  • Armando A. Rodríguez
  • David E. Williams
  • Patricia Lorenzo-Luaces
  • Olga Valdés
  • Yasnay Hernandez
Original Article

DOI: 10.1007/s10126-008-9123-8

Cite this article as:
Regalado, E.L., Rodríguez, M., Menéndez, R. et al. Mar Biotechnol (2009) 11: 74. doi:10.1007/s10126-008-9123-8

Abstract

Daily topical application of the aqueous ethanolic extract of the marine sea grass, Thalassia testudinum, on mice skin exposed to UVB radiation resulted in a dose-dependent recovery of the skin macroscopic alterations over a 6-day period. Maximal effect (90%) occurred at a dose of 240 μg/cm2, with no additional effects at higher doses. Bioassay-guided fractionation of the plant extract resulted in the isolation of thalassiolin B (1). Topical application of 1 (240 μg/cm2) markedly reduces skin UVB-induced damage. In addition, thalassiolin B scavenged 2,2-diphenyl-2-picrylhydrazyl radical with an EC50 = 100 μg/ml. These results suggest that thalassiolin B is responsible for the skin-regenerating effects of the crude extract of T. testudinum.

Keywords

Flavonoids Thalassiolin B DPPH scavenged Antioxidant activity Skin regenerating activity Thalassia testudinum 

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Erik L. Regalado
    • 1
  • María Rodríguez
    • 2
  • Roberto Menéndez
    • 3
  • Ángel A. Concepción
    • 4
  • Clara Nogueiras
    • 5
  • Abilio Laguna
    • 1
  • Armando A. Rodríguez
    • 1
  • David E. Williams
    • 6
  • Patricia Lorenzo-Luaces
    • 2
  • Olga Valdés
    • 1
  • Yasnay Hernandez
    • 1
  1. 1.Department of ChemistryCenter of Marine BioproductsHavanaCuba
  2. 2.Department of Chemistry, Laboratory of CosmeticsCenter of Marine BioproductsHavanaCuba
  3. 3.Department of PharmacologyCenter of Marine BioproductsHavanaCuba
  4. 4.Department of HistologyNational Center of Medical GeneticHavanaCuba
  5. 5.Department of Natural Products, Faculty of ChemistryUniversity of HavanaHavanaCuba
  6. 6.Department of ChemistryUniversity of British ColumbiaVancouverCanada