Marine Biotechnology

, Volume 9, Issue 4, pp 479–490

Protective Effect of Enzymatic Extracts from Microalgae Against DNA Damage Induced by H2O2

Authors

  • Rohan Karawita
    • Faculty of Applied Marine ScienceCheju National University
  • Mahinda Senevirathne
    • Department of Food Science and EngineeringCheju National University
  • Yasantha Athukorala
    • Faculty of Applied Marine ScienceCheju National University
  • Abu Affan
    • Department of OceanographyCheju National University
  • Young-Jae Lee
    • Deparment of Veterinary ScienceCheju National University
  • Se-Kwon Kim
    • Department of ChemistryPukyong National University
    • Department of OceanographyCheju National University
  • You-Jin Jeon
    • Faculty of Applied Marine ScienceCheju National University
    • Radiological Science Research InstituteCheju National University
Original Article

DOI: 10.1007/s10126-007-9007-3

Cite this article as:
Karawita, R., Senevirathne, M., Athukorala, Y. et al. Mar Biotechnol (2007) 9: 479. doi:10.1007/s10126-007-9007-3

Abstract

The enzymatic extracts from seven species of microalgae (Pediastrum duplex, Dactylococcopsis fascicularis, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habitats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated for their antioxidant activity. Of the extracts tested, the AMG 300 L (an exo 1, 4-α-d-glucosidase) extract of P. duplex, the Viscozyme extract of Navicula sp., and the Celluclast extract of A. longipes provided the most potential as antioxidants. Meanwhile, the Termamyl extract of P. duplex in an H2O2 scavenging assay exhibited an approximate 60% scavenging effect. In this study, we report that the DNA damage inhibitory effects of P. duplex (Termamyl extract) and D. fascicularis (Kojizyme extract) were nearly 80% and 69% respectively at a concentration of 100 μg/ml. Thus, it is suggested that the microalgae tested in this study yield promising DNA damage inhibitory properties on mouse lymphoma L 5178 cells that are treated with H2O2. Therefore, microalgae such as P. duplex may be an excellent source of naturally occurring antioxidant compounds with potent DNA damage inhibition potential.

Keywords

antioxidant activityDNA damageenzymatic extractsH2O2microalgae scavenging activity

Copyright information

© Springer Science+Business Media, LLC 2007