Original Article

Marine Biotechnology

, Volume 9, Issue 4, pp 479-490

First online:

Protective Effect of Enzymatic Extracts from Microalgae Against DNA Damage Induced by H2O2

  • Rohan KarawitaAffiliated withFaculty of Applied Marine Science, Cheju National University
  • , Mahinda SenevirathneAffiliated withDepartment of Food Science and Engineering, Cheju National University
  • , Yasantha AthukoralaAffiliated withFaculty of Applied Marine Science, Cheju National University
  • , Abu AffanAffiliated withDepartment of Oceanography, Cheju National University
  • , Young-Jae LeeAffiliated withDeparment of Veterinary Science, Cheju National University
  • , Se-Kwon KimAffiliated withDepartment of Chemistry, Pukyong National University
  • , Joon-Baek LeeAffiliated withDepartment of Oceanography, Cheju National University Email author 
  • , You-Jin JeonAffiliated withFaculty of Applied Marine Science, Cheju National UniversityRadiological Science Research Institute, Cheju National University

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Abstract

The enzymatic extracts from seven species of microalgae (Pediastrum duplex, Dactylococcopsis fascicularis, Halochlorococcum porphyrae, Oltmannsiellopsis unicellularis, Achnanthes longipes, Navicula sp. and Amphora coffeaeformis) collected from three habitats (freshwater, tidal pool, and coastal benthic) at Jeju Island in Korea were investigated for their antioxidant activity. Of the extracts tested, the AMG 300 L (an exo 1, 4-α-d-glucosidase) extract of P. duplex, the Viscozyme extract of Navicula sp., and the Celluclast extract of A. longipes provided the most potential as antioxidants. Meanwhile, the Termamyl extract of P. duplex in an H2O2 scavenging assay exhibited an approximate 60% scavenging effect. In this study, we report that the DNA damage inhibitory effects of P. duplex (Termamyl extract) and D. fascicularis (Kojizyme extract) were nearly 80% and 69% respectively at a concentration of 100 μg/ml. Thus, it is suggested that the microalgae tested in this study yield promising DNA damage inhibitory properties on mouse lymphoma L 5178 cells that are treated with H2O2. Therefore, microalgae such as P. duplex may be an excellent source of naturally occurring antioxidant compounds with potent DNA damage inhibition potential.

Keywords

antioxidant activity DNA damage enzymatic extracts H2O2 microalgae scavenging activity