Marine Biotechnology

, Volume 9, Issue 3, pp 388–397

Novel Antifoulants: Inhibition of Larval Attachment by Proteases

  • Sergey Dobretsov
  • Hairong Xiong
  • Ying Xu
  • Lisa A. Levin
  • Pei-Yuan Qian
Original Article

DOI: 10.1007/s10126-007-7091-z

Cite this article as:
Dobretsov, S., Xiong, H., Xu, Y. et al. Mar Biotechnol (2007) 9: 388. doi:10.1007/s10126-007-7091-z

Abstract

We investigated the effect of commercially available enzymes (α-amylase, α-galactosidase, papain, trypsin, and lipase) as well as proteases from deep-sea bacteria on the larval attachment of the bryozoan Bugula neritina L. The 50% effective concentrations (EC50) of the commercial proteases were 10 times lower than those of other enzymes. Crude proteases from six deep-sea Pseudoalteromonas species significantly decreased larval attachment at concentrations of 0.03 to 1 mIU ml−1. The EC50 of the pure protease from the bacterium Pseudoalteromonas issachenkonii UST041101-043 was close to 1 ng ml−1 (0.1 mIU ml−1). The protease and trypsin individually incorporated in a water-soluble paint matrix inhibited biofouling in a field experiment. There are certain correlations between production of proteases by bacterial films and inhibition of larval attachment. None of the bacteria with biofilms that induced attachment of B. neritina produced proteolytic enzymes, whereas most of the bacteria that formed inhibitive biofilms produced proteases. Our investigation demonstrated the potential use of proteolytic enzymes for antifouling defense.

Keywords

antifoulingdeep-sea bacteriaenzymeslarval attachmentproteasessettlement

Copyright information

© Springer Science + Business Media, LLC 2007

Authors and Affiliations

  • Sergey Dobretsov
    • 1
    • 2
  • Hairong Xiong
    • 1
  • Ying Xu
    • 1
  • Lisa A. Levin
    • 3
  • Pei-Yuan Qian
    • 1
  1. 1.Coastal Marine Laboratory, Department of BiologyHong Kong University of Science and TechnologyKowloonPR China
  2. 2.IFM-GEOMARKiel UniversityKielGermany
  3. 3.Integrative Oceanography DivisionScripps Institution of OceanographyLa JollaUSA