Original Article

Marine Biotechnology

, Volume 8, Issue 1, pp 27-39

Open Access This content is freely available online to anyone, anywhere at any time.

Isolation and Culture of a Marine Bacterium Degrading the Sulfated Fucans from Marine Brown Algae

  • Valérie DescampsAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie)
  • , Sébastien ColinAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie)
  • , Marc LahayeAffiliated withleurs Organisations et Interactions, INRA, Unité de Recherche sur les Polysaccharides
  • , Murielle JamAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie)
  • , Christophe RichardAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie)
  • , Philippe PotinAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie)
  • , Tristan BarbeyronAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie) Email author 
  • , Jean-Claude YvinAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie)
  • , Bernard KloaregAffiliated withUMR 7139 (CNRS, Laboratoires Goëmar and Université Pierre et Marie Curie)

Abstract

Fucoidans are matrix polysaccharides from marine brown algae, consisting of an α-l-fucose backbone substituted by sulfate-ester groups and masked with ramifications containing other monosaccharide residues. In spite of their interest as biologically active compounds in a number of homologous and heterologous systems, no convenient sources with fucanase activity are available yet for the degradation of the fucalean algae. We here report on the isolation, characterization, and culture conditions of a bacterial strain capable of degrading various brown algal fucoidans. This bacterium, a member of the family Flavobacteriaceae, was shown to secrete fucoidan endo-hydrolase activity. An extracellular enzyme preparation was used to degrade the fucoidan from the brown alga Pelvetia canaliculata. End products included a tetrasaccharide and a hexasaccharide made of the repetition of disaccharidic units consisting of α-1→3-l-fucopyranose-2-sulfate-α-1→4-l-fucopyranose-2,3-disulfate, with the 3-linked residues at the nonreducing end.

Keywords

Flavobacteriaceae fucalean brown algae fucan oligosaccharides fucoidan fucoidanase marine bacteria